Differential phosphoproteome profiling reveals a functional role for VASP in Helicobacter pylori-induced cytoskeleton turnover in gastric epithelial cells
Knauer O, Binai NA, Carra G, Beckhaus T, Hanschmann KM, Renne T, Backert S, Karas M, Wessler S (2008)
Publication Type: Journal article
Publication year: 2008
Journal
Book Volume: 10
Pages Range: 2285-2296
Journal Issue: 11
DOI: 10.1111/j.1462-5822.2008.01207.x
Abstract
Infection with Helicobacter pylori induces various gastric diseases, including ulceration, gastritis and neoplasia. As H. pylori-induced cellular mechanisms leading to these disease states are widely unclear, we analysed the phosphoproteome of H. pylori-infected gastric epithelial cells. Phosphoproteins from infected cells were enriched using affinity columns and analysed by two-dimensional gel electrophoresis and mass spectrometry. Eleven novel phosphoproteins that showed differentially regulated phosphorylation levels during H. pylori infection were identified. Interestingly, the identified proteins were actin-binding, transport and folding, RNA/DNA-binding or cancer-associated proteins. We analysed functions of one identified H. pylori-regulated candidate, the vasodilator-stimulated phosphoprotein (VASP). H. pylori induced VASP phosphorylation at residues Ser157, Ser239 and Thr278, which was enhanced by the bacterial oncogene cytotoxin-associated gene A. Overexpression of a phosphorylation-resistant VASP mutant efficiently blocked host cell elongation. We identified cGMP-dependent protein kinase G-mediated Ser239 and Thr278 phosphorylation of VASP as a crucial event in H. pylori-dependent host cell elongation. These results suggest that phosphorylated VASP could be a novel target candidate for therapeutic intervention in H. pylori-related gastric diseases. © 2008 The Authors Journal compilation © 2008 Blackwell Publishing Ltd.
Authors with CRIS profile
Involved external institutions
Paul Ehrlich Institute / Paul-Ehrlich-Institut – Bundesinstitut für Impfstoffe und biomedizinische Arzneimittel (PEI)
Germany (DE)
Goethe-Universität Frankfurt am Main
Germany (DE)
Julius-Maximilians-Universität Würzburg
Germany (DE)
Otto-von-Guericke-Universität Magdeburg
Germany (DE)
Germany (DE)
Goethe-Universität Frankfurt am Main
Germany (DE)
Julius-Maximilians-Universität Würzburg
Germany (DE)
Otto-von-Guericke-Universität Magdeburg
Germany (DE)
How to cite
APA:
Knauer, O., Binai, N.A., Carra, G., Beckhaus, T., Hanschmann, K.-M., Renne, T.,... Wessler, S. (2008). Differential phosphoproteome profiling reveals a functional role for VASP in Helicobacter pylori-induced cytoskeleton turnover in gastric epithelial cells. Cellular Microbiology, 10(11), 2285-2296. https://doi.org/10.1111/j.1462-5822.2008.01207.x
MLA:
Knauer, Olivia, et al. "Differential phosphoproteome profiling reveals a functional role for VASP in Helicobacter pylori-induced cytoskeleton turnover in gastric epithelial cells." Cellular Microbiology 10.11 (2008): 2285-2296.
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