Live Helicobacter pylori in the root canal of endodontic-infected deciduous teeth
Hirsch C, Tegtmeyer N, Rohde M, Rowland M, Oyarzabal OA, Backert S (2012)
Publication Type: Journal article
Publication year: 2012
Journal
Book Volume: 47
Pages Range: 936-940
Journal Issue: 8
DOI: 10.1007/s00535-012-0618-8
Abstract
Background: Many polymerase chain reaction (PCR)- based studies have shown that Helicobacter pylori DNA is prevalent in the oral cavity, but reports on the isolation of live bacteria are extremely rare. Thus, it is still unclear whether H. pylori can indeed survive in the oral environment. Methods: Here we used electron microscopy, selective growth techniques, urease assays, 16S rRNA PCR, and western blotting to investigate the possible presence of live H. pylori in 10 root canal and corresponding plaque samples of endodontic-infected deciduous teeth in three children. Results: Although H. pylori DNA was verifiable by PCR in several plaque and root canal samples, bacterial colonies could only be grown from two root canals, but not from plaque. These colonies were unequivocally identified as H. pylori by microscopic, genetic, and biochemical approaches. Conclusions: Our findings show that root canals of endodontic- infected teeth may be a reservoir for live H. pylori that could serve as a potential source for transmission. © Springer 2012.
Authors with CRIS profile
Involved external institutions
Helmholtz-Zentrum für Infektionsforschung (HZI)
Germany (DE)
Universität Leipzig
Germany (DE)
University College Dublin (UCD)
Ireland (IE)
IEH Laboratories and Consulting Group
United States (USA) (US)
Germany (DE)
Universität Leipzig
Germany (DE)
University College Dublin (UCD)
Ireland (IE)
IEH Laboratories and Consulting Group
United States (USA) (US)
How to cite
APA:
Hirsch, C., Tegtmeyer, N., Rohde, M., Rowland, M., Oyarzabal, O.A., & Backert, S. (2012). Live Helicobacter pylori in the root canal of endodontic-infected deciduous teeth. Journal of Gastroenterology, 47(8), 936-940. https://doi.org/10.1007/s00535-012-0618-8
MLA:
Hirsch, Christian, et al. "Live Helicobacter pylori in the root canal of endodontic-infected deciduous teeth." Journal of Gastroenterology 47.8 (2012): 936-940.
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