Peptidoglycan maturation enzymes affect flagellar functionality in bacteria
Roure S, Bonis M, Chaput C, Ecobichon C, Mattox A, Barriere C, Geldmacher N, Guadagnini S, Schmitt C, Prevost MC, Labigne A, Backert S, Ferrero RL, Boneca IG (2012)
Publication Type: Journal article
Publication year: 2012
Journal
Book Volume: 86
Pages Range: 845-856
Journal Issue: 4
DOI: 10.1111/mmi.12019
Abstract
The flagellar machinery is a highly complex organelle composed of a free rotating flagellum and a fixed stator that converts energy into movement. The assembly of the flagella and the stator requires interactions with the peptidoglycan layer through which the organelle has to pass for externalization. Lytic transglycosylases are peptidoglycan degrading enzymes that cleave the sugar backbone of peptidoglycan layer. We show that an endogenous lytic transglycosylase is required for full motility ofHelicobacter pylori and colonization of the gastric mucosa. Deficiency of motility resulted from a paralysed phenotype implying an altered ability to generate flagellar rotation. Similarly, another Gram-negative pathogen Salmonella typhimurium and the Gram-positive pathogen Listeria monocytogenes required the activity of lytic transglycosylases, Slt or MltC, and a glucosaminidase (Auto), respectively,for full motility. Furthermore, we show that in absence of the appropriate lytic transglycosylase theflagellar motor protein MotB from H. pylori does not localize properly to the bacterial pole.We present a new model involving the maturation of the surrounding peptidoglycan for the proper anchoring and functionality of the flagellar motor. © 2012 Blackwell Publishing Ltd.
Authors with CRIS profile
Involved external institutions
Institut Pasteur
France (FR)
Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. (MPG) / Max Planck Society for the Advancement of Science
Germany (DE)
University College Dublin (UCD)
Ireland (IE)
France (FR)
Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. (MPG) / Max Planck Society for the Advancement of Science
Germany (DE)
University College Dublin (UCD)
Ireland (IE)
How to cite
APA:
Roure, S., Bonis, M., Chaput, C., Ecobichon, C., Mattox, A., Barriere, C.,... Boneca, I.G. (2012). Peptidoglycan maturation enzymes affect flagellar functionality in bacteria. Molecular Microbiology, 86(4), 845-856. https://doi.org/10.1111/mmi.12019
MLA:
Roure, Sophie, et al. "Peptidoglycan maturation enzymes affect flagellar functionality in bacteria." Molecular Microbiology 86.4 (2012): 845-856.
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