High-resolution reconstruction of the beating zebrafish heart
Mickoleit M, Schmid B, Weber M, Fahrbach FO, Hombach S, Reischauer S, Huisken J (2014)
Publication Type: Journal article
Publication year: 2014
Journal
Book Volume: 11
Pages Range: 919-922
Journal Issue: 9
DOI: 10.1038/nmeth.3037
Abstract
The heart's continuous motion makes it difficult to capture high-resolution images of this organ in vivo. We developed tools based on high-speed selective plane illumination microscopy (SPIMIM), offering pristine views into the beating zebrafish heart. We captured three-dimensional cardiac dynamics with postacquisition synchronization of multiview movie stacks, obtained static high-resolution reconstructions by briefly stopping the heart with optogenetics and resolved nonperiodic phenomena by high-speed volume scanning with a liquid lens.
Authors with CRIS profile
Involved external institutions
Max-Planck-Institut für molekulare Zellbiologie und Genetik / Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Germany (DE)
Max Planck Institute for Heart and Lung Research / Max-Planck-Institut für Herz- und Lungenforschung
Germany (DE)
Germany (DE)
Max Planck Institute for Heart and Lung Research / Max-Planck-Institut für Herz- und Lungenforschung
Germany (DE)
How to cite
APA:
Mickoleit, M., Schmid, B., Weber, M., Fahrbach, F.O., Hombach, S., Reischauer, S., & Huisken, J. (2014). High-resolution reconstruction of the beating zebrafish heart. Nature Methods, 11(9), 919-922. https://dx.doi.org/10.1038/nmeth.3037
MLA:
Mickoleit, Michaela, et al. "High-resolution reconstruction of the beating zebrafish heart." Nature Methods 11.9 (2014): 919-922.
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