Bassi A, Schmid B, Huisken J (2015)
Publication Type: Journal article
Publication year: 2015
Book Volume: 142
Pages Range: 1016-1020
Journal Issue: 5
DOI: 10.1242/dev.116970
Fluorescently labeled structures can be spectrally isolated and imaged at high resolution in living embryos by light sheet microscopy. Multimodal imaging techniques are now needed to put these distinct structures back into the context of the surrounding tissue.We found that the bright-field contrast of unstained specimens in a selective plane illumination microscopy (SPIM) setup can be exploited for in vivo tomographic reconstructions of the threedimensional anatomy of zebrafish, without causing phototoxicity. We report multimodal imaging of entire zebrafish embryos over several hours of development, as well as segmentation, tracking and automatic registration of individual organs.
APA:
Bassi, A., Schmid, B., & Huisken, J. (2015). Optical tomography complements light sheet microscopy for in toto imaging of zebrafish development. Development, 142(5), 1016-1020. https://dx.doi.org/10.1242/dev.116970
MLA:
Bassi, Andrea, Benjamin Schmid, and Jan Huisken. "Optical tomography complements light sheet microscopy for in toto imaging of zebrafish development." Development 142.5 (2015): 1016-1020.
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