Hyperspectral light sheet microscopy

Jahr W, Schmid B, Schmied C, Fahrbach FO, Huisken J (2015)

Publication Type: Journal article

Publication year: 2015

Journal

Nature Communications Nature Publishing Group: Nature Communications

Book Volume: 6

Article Number: 7990

DOI: 10.1038/ncomms8990

Abstract

To study the development and interactions of cells and tissues, multiple fluorescent markers need to be imaged efficiently in a single living organism. Instead of acquiring individual colours sequentially with filters, we created a platform based on line-scanning light sheet microscopy to record the entire spectrum for each pixel in a three-dimensional volume. We evaluated data sets with varying spectral sampling and determined the optimal channel width to be around 5 nm. With the help of these data sets, we show that our setup outperforms filter-based approaches with regard to image quality and discrimination of fluorophores. By spectral unmixing we resolved overlapping fluorophores with up to nanometre resolution and removed autofluorescence in zebrafish and fruit fly embryos.

Authors with CRIS profile

Benjamin Schmid

Involved external institutions

Max-Planck-Institut für molekulare Zellbiologie und Genetik / Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG) DE Germany (DE)

How to cite

APA:

Jahr, W., Schmid, B., Schmied, C., Fahrbach, F.O., & Huisken, J. (2015). Hyperspectral light sheet microscopy. Nature Communications, 6. https://dx.doi.org/10.1038/ncomms8990

MLA:

Jahr, Wiebke, et al. "Hyperspectral light sheet microscopy." Nature Communications 6 (2015).

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