Recombinant spider silk protein eADF4(C16)-RGD coatings are suitable for cardiac tissue engineering

Kramer JP, Aigner TB, Petzold J, Roshanbinfar K, Scheibel T, Engel F (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Scientific Reports Nature Publishing Group: Open Access Journals - Option B

Book Volume: 10

Article Number: 8789

Journal Issue: 1

DOI: 10.1038/s41598-020-65786-4

Abstract

Cardiac tissue engineering is a promising approach to treat cardiovascular diseases, which are a major socio-economic burden worldwide. An optimal material for cardiac tissue engineering, allowing cardiomyocyte attachment and exhibiting proper immunocompatibility, biocompatibility and mechanical characteristics, has not yet emerged. An additional challenge is to develop a fabrication method that enables the generation of proper hierarchical structures and constructs with a high density of cardiomyocytes for optimal contractility. Thus, there is a focus on identifying suitable materials for cardiac tissue engineering. Here, we investigated the interaction of neonatal rat heart cells with engineered spider silk protein (eADF4(C16)) tagged with the tripeptide arginyl-glycyl-aspartic acid cell adhesion motif RGD, which can be used as coating, but can also be 3D printed. Cardiomyocytes, fibroblasts, and endothelial cells attached well to eADF4(C16)-RGD coatings, which did not induce hypertrophy in cardiomyocytes, but allowed response to hypertrophic as well as proliferative stimuli. Furthermore, Kymograph and MUSCLEMOTION analyses showed proper cardiomyocyte beating characteristics on spider silk coatings, and cardiomyocytes formed compact cell aggregates, exhibiting markedly higher speed of contraction than cardiomyocyte mono-layers on fibronectin. The results suggest that eADF4(C16)-RGD is a promising material for cardiac tissue engineering.

Authors with CRIS profile

Kaveh Roshanbinfar Professur für Experimentelle Nieren- und Kreislaufforschung Felix Engel Professur für Experimentelle Nieren- und Kreislaufforschung

Involved external institutions

Universität Bayreuth DE Germany (DE)

How to cite

APA:

Kramer, J.P., Aigner, T.B., Petzold, J., Roshanbinfar, K., Scheibel, T., & Engel, F. (2020). Recombinant spider silk protein eADF4(C16)-RGD coatings are suitable for cardiac tissue engineering. Scientific Reports, 10(1). https://doi.org/10.1038/s41598-020-65786-4

MLA:

Kramer, Johannes P.M., et al. "Recombinant spider silk protein eADF4(C16)-RGD coatings are suitable for cardiac tissue engineering." Scientific Reports 10.1 (2020).

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