DNA stool test for colorectal cancer: hypermethylation of the secreted frizzled-related protein-1 gene.

Zhang W, Bauer M, Croner RS, Pelz JOW, Lodygin D, Hermeking H, Stürzl M, Hohenberger W, Matzel K (2007)

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2007

Journal

Diseases of the colon & rectum Lippincott, Williams & Wilkins

Book Volume: 50

Pages Range: 1618-26; discussion 1626-7

Journal Issue: 10

DOI: 10.1007/s10350-007-0286-6

Abstract

To investigate a potential mode of noninvasive screening for colorectal cancer, we evaluated the hypermethylation of the secreted frizzled-related protein-1 gene promoter in human stool DNA.\nIn stool samples from 36 patients with colorectal neoplasia (7 adenoma, 29 colorectal cancer) and 17 healthy control subjects, isolated DNA was treated with sodium bisulfite and analyzed by methylation-specific polymerase chain reaction with primers specific for methylated or unmethylated promoter sequences of the secreted frizzled-related protein-1 gene.\nHypermethylation of the secreted frizzled-related protein-1 promoter was present in the stool DNA of patients with adenoma and colorectal cancer. A sensitivity of 89 percent and specificity of 86 percent were achieved in the detection of colorectal neoplasia. The difference in hypermethylation status of the secreted frizzled-related protein-1 promoter between the patients with colorectal neoplasia and the control group was statistically highly significant (P < 0.001). Adenoma and early tumor Stage I (International Union Against Cancer) displayed both unmethylated and methylated secreted frizzled-related protein-1 promoter sequences, whereas advanced tumor stages showed only methylated secreted frizzled-related protein-1 (P = 0.05).\nThe results indicate that this DNA stool test of hypermethylation of the secreted frizzled-related protein-1 promoter is a sensitive and specific method. It has the potential of a clinically useful test for the early detection of colorectal cancer.\nPURPOSE\nMETHODS\nRESULTS\nCONCLUSIONS

Authors with CRIS profile

Michael Stürzl Professur für Molekulare und Experimentelle Chirurgie Werner Hohenberger Department of Surgery Klaus Matzel Lehrstuhl für Allgemein- und Viszeralchirurgie

Involved external institutions

Max-Planck-Institut für Biochemie / Max Planck Institute of Biochemistry DE Germany (DE)

How to cite

APA:

Zhang, W., Bauer, M., Croner, R.S., Pelz, J.O.W., Lodygin, D., Hermeking, H.,... Matzel, K. (2007). DNA stool test for colorectal cancer: hypermethylation of the secreted frizzled-related protein-1 gene. Diseases of the colon & rectum, 50(10), 1618-26; discussion 1626-7. https://doi.org/10.1007/s10350-007-0286-6

MLA:

Zhang, Wei, et al. "DNA stool test for colorectal cancer: hypermethylation of the secreted frizzled-related protein-1 gene." Diseases of the colon & rectum 50.10 (2007): 1618-26; discussion 1626-7.

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