The Ebola virus glycoprotein and HIV-1 Vpu employ different strategies to counteract the antiviral factor tetherin.

Kühl A, Banning C, Marzi A, Votteler J, Steffen I, Bertram S, Glowacka I, Konrad A, Stürzl M, Guo JT, Schubert U, Feldmann H, Behrens G, Schindler M, Pöhlmann S (2011)

Publication Status: Published

Publication Type: Journal article

Publication year: 2011

Journal

Journal of Infectious Diseases University of Chicago Press / Oxford University Press (OUP): Policy A1 - Oxford Open Option C

Book Volume: 204 Suppl 3

Pages Range: S850-60

DOI: 10.1093/infdis/jir378

Abstract

The antiviral protein tetherin/BST2/CD317/HM1.24 restricts cellular egress of human immunodeficiency virus (HIV) and of particles mimicking the Ebola virus (EBOV), a hemorrhagic fever virus. The HIV-1 viral protein U (Vpu) and the EBOV-glycoprotein (EBOV-GP) both inhibit tetherin. Here, we compared tetherin counteraction by EBOV-GP and Vpu. We found that EBOV-GP but not Vpu counteracted tetherin from different primate species, indicating that EBOV-GP and Vpu target tetherin differentially. Tetherin interacted with the GP2 subunit of EBOV-GP, which might encode the determinants for tetherin counteraction. Vpu reduced cell surface expression of tetherin while EBOV-GP did not, suggesting that both proteins employ different mechanisms to counteract tetherin. Finally, Marburg virus (MARV)-GP also inhibited tetherin and downregulated tetherin in a cell type-dependent fashion, indicating that tetherin antagonism depends on the cellular source of tetherin. Collectively, our results indicate that EBOV-GP counteracts tetherin by a novel mechanism and that tetherin inhibition is conserved between EBOV-GP and MARV-GP.

Authors with CRIS profile

Michael Stürzl Professur für Molekulare und Experimentelle Chirurgie Ulrich Schubert Professur für Virologie

Involved external institutions

Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich DE Germany (DE)

How to cite

APA:

Kühl, A., Banning, C., Marzi, A., Votteler, J., Steffen, I., Bertram, S.,... Pöhlmann, S. (2011). The Ebola virus glycoprotein and HIV-1 Vpu employ different strategies to counteract the antiviral factor tetherin. Journal of Infectious Diseases, 204 Suppl 3, S850-60. https://doi.org/10.1093/infdis/jir378

MLA:

Kühl, Annika, et al. "The Ebola virus glycoprotein and HIV-1 Vpu employ different strategies to counteract the antiviral factor tetherin." Journal of Infectious Diseases 204 Suppl 3 (2011): S850-60.

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