Adult and cord blood-derived high-affinity gB-CAR-T cells effectively react against human cytomegalovirus infections

Olbrich H, Theobald SJ, Slabik C, Gerasch L, Schneider A, Mach M, Shum T, Mamonkin M, Stripecke R (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Human Gene Therapy Mary Ann Liebert

Book Volume: 31

Pages Range: 423-439

Journal Issue: 7-8

DOI: 10.1089/hum.2019.149

Abstract

Human cytomegalovirus (HCMV) reactivations are associated with lower overall survival after transplantations. Adoptive transfer of HCMV-reactive expanded or selected T cells can be applied as a compassionate use, but requires that the human leukocyte antigen-matched donor provides memory cells against HCMV. To overcome this, we developed engineered T cells expressing chimeric antigen receptors (CARs) targeted against the HCMV glycoprotein B (gB) expressed upon viral reactivation. Single-chain variable fragments (scFvs) derived from a human high-affinity gB-specific neutralizing monoclonal antibody (SM5-1) were fused to CARs with 4-1BB (BBL) or CD28 (28S) costimulatory domains and subcloned into retroviral vectors. CD4+ and CD8+ T cells obtained from HCMV-seronegative adult blood or cord blood (CB) transduced with the vectors efficiently expressed the gB-CARs. The specificity and potency of gB-CAR-T cells were demonstrated and compared in vitro using the following: 293T cells expressing gB, and with mesenchymal stem cells infected with a HCMV TB40 strain expressing Gaussia luciferase (HCMV/GLuc). BBL-gB-CAR-T cells generated with adult or CB demonstrated significantly higher in vitro activation and cytotoxicity performance than 28-gB-CAR-T cells. Nod.Rag.Gamma (NRG) mice transplanted with human CB CD34+ cells with long-term human immune reconstitution were used to model HCMV/GLuc infection in vivo by optical imaging analyses. One week after administration, response to BBL-gB-CAR-T cell therapy was observed for 5/8 mice, defined by significant reduction of the bioluminescent signal in relation to untreated controls. Response to therapy was sporadically associated with CAR detection in spleen. Thus, exploring scFv derived from the high-affinity gB-antibody SM5-1 and the 4-1BB signaling domain for CAR design enabled an in vitro high on-target effect and cytotoxicity and encouraging results in vivo. Therefore, gB-CAR-T cells can be a future clinical option for treatment of HCMV reactivations, particularly when memory T cells from the donors are not available.

Authors with CRIS profile

Michael Mach Medizinische Fakultät

Additional Organisation(s)

Institute of Clinical and Molecular Virology

Involved external institutions

Medizinische Hochschule Hannover (MHH) / Hannover Medical School DE Germany (DE) Baylor College of Medicine US United States (USA) (US)

How to cite

APA:

Olbrich, H., Theobald, S.J., Slabik, C., Gerasch, L., Schneider, A., Mach, M.,... Stripecke, R. (2020). Adult and cord blood-derived high-affinity gB-CAR-T cells effectively react against human cytomegalovirus infections. Human Gene Therapy, 31(7-8), 423-439. https://doi.org/10.1089/hum.2019.149

MLA:

Olbrich, Henning, et al. "Adult and cord blood-derived high-affinity gB-CAR-T cells effectively react against human cytomegalovirus infections." Human Gene Therapy 31.7-8 (2020): 423-439.

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