Trimethylamine-N-oxide (TMAO) determined by LC-MS/MS: Distribution and correlates in the population-based PopGen cohort
Geßner A, Di Giuseppe R, Koch M, Fromm M, Lieb W, Maas R (2020)
Publication Type: Journal article
Publication year: 2020
Journal
Abstract
Accumulating evidence indicates that trimethylamine-N-oxide (TMAO) may play a causal role in cardiovascular disease (CVD), chronic kidney disease (CKD) and type 2 diabetes (T2D). TMAO plasma concentrations show considerable intra- A nd inter-individual variation, underscoring the need for a reference interval in the general population to identify elevated TMAO concentrations. TMAO concentrations were determined using an LC-MS/MS assay in a community-based sample of the PopGen control cohort consisting of 694 participants (54% men; aged 25-82 years) free of clinical CVD, CKD and T2D. We defined reference intervals for TMAO concentrations in human plasma using the 2.5th and 97.5th percentiles. Using multivariable regression analysis we analyzed the association of estimated glomerular filtration rate (EGFR), sex, and dietary intake and TMAO plasma concentrations. TMAO plasma concentrations were positively skewed and differed by sex. The median TMAO plasma concentration in men was 3.91 (Q1-Q3: 2.87-6.10) μmol/L and the reference interval 1.28-19.67 μmol/L (2.5th-97.5th percentile). In women median TMAO plasma concentration was 3.56 (Q1-Q3: 2.41-5.15) μmol/L and the reference interval 1.08-17.12 μmol/L. In multivariable regression analysis plasma TMAO was associated with sex, renal function and diet. The association of TMAO and diet was significant for intake of fish and shellfish in men only. In a community-based sample free of apparent CVD and renal disease, we report the distribution of TMAO plasma concentrations with sex, renal function and diet as factors associated with plasma TMAO, and suggest reference intervals. These data may facilitate standardized comparisons of TMAO across populations.
Authors with CRIS profile
Arne Geßner
Lehrstuhl für Klinische Pharmakologie und Klinische Toxikologie
Martin Fromm
Lehrstuhl für Klinische Pharmakologie und Klinische Toxikologie
Renke Maas
Professur für Arzneimitteltherapiesicherheit und Klinische Pharmakologie
Involved external institutions
Germany (DE)How to cite
APA:
Geßner, A., Di Giuseppe, R., Koch, M., Fromm, M., Lieb, W., & Maas, R. (2020). Trimethylamine-N-oxide (TMAO) determined by LC-MS/MS: Distribution and correlates in the population-based PopGen cohort. Clinical Chemistry and Laboratory Medicine. https://doi.org/10.1515/cclm-2019-1146
MLA:
Geßner, Arne, et al. "Trimethylamine-N-oxide (TMAO) determined by LC-MS/MS: Distribution and correlates in the population-based PopGen cohort." Clinical Chemistry and Laboratory Medicine (2020).
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