Inhibition of stress fiber formation preserves blood-brain barrier after intracerebral hemorrhage in mice
Manaenko A, Yang P, Nowrangi D, Budbazar E, Hartman RE, Obenaus A, Pearce WJ, Zhang JH, Tang J (2018)
Publication Type: Journal article
Publication year: 2018
Journal
Book Volume: 38
Pages Range: 87-102
Journal Issue: 1
Abstract
Intracerebral hemorrhage (ICH) represents the deadliest subtype of all strokes. The development of brain edema, a consequence of blood-brain barrier (BBB) disruption, is the most life-threatening event after ICH. Pathophysiological conditions activate the endothelium, one of the components of BBB, inducing rearrangement of the actin cytoskeleton. Upon activation, globular actin assembles into a filamentous actin resulting in the formation of contractile actin bundles, stress fibers. The contraction of stress fibers leads to the formation of intercellular gaps between endothelial cells increasing the permeability of BBB. In the present study, we investigated the effect of ICH on stress fiber formation in CD1 mice. We hypothesized that ICH-induced formation of stress fiber is triggered by the activation of PDGFR-β and mediated by the cortactin/RhoA/LIMK pathway. We demonstrated that ICH induces formation of stress fibers. Furthermore, we demonstrated that the inhibition of PDGFR-β and its downstream reduced the number of stress fibers, preserving BBB and resulting in the amelioration of brain edema and improvement of neurological functions in mice after ICH.
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United States (USA) (US)How to cite
APA:
Manaenko, A., Yang, P., Nowrangi, D., Budbazar, E., Hartman, R.E., Obenaus, A.,... Tang, J. (2018). Inhibition of stress fiber formation preserves blood-brain barrier after intracerebral hemorrhage in mice. Journal of Cerebral Blood Flow and Metabolism, 38(1), 87-102. https://dx.doi.org/10.1177/0271678X16679169
MLA:
Manaenko, Anatol, et al. "Inhibition of stress fiber formation preserves blood-brain barrier after intracerebral hemorrhage in mice." Journal of Cerebral Blood Flow and Metabolism 38.1 (2018): 87-102.
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