A 17-kDa Fragment of Lactoferrin Associates With the Termination of Inflammation and Peptides Within Promote Resolution
Lutaty A, Soboh S, Schif-Zuck S, Zeituni-Timor O, Rostoker R, Podolska M, Herrmann M, Munoz LE, Ariel A, Schauer C (2018)
Publication Type: Journal article
Publication year: 2018
Journal
Book Volume: 9
Pages Range: 77-77
Abstract
During the resolution of inflammation, macrophages engulf apoptotic polymorphonuclear cells (PMN) and can accumulate large numbers of their corpses. Here, we report that resolution phase macrophages acquire the neutrophil-derived glycoprotein lactoferrin (Lf) and fragments thereof in vivo and ex vivo. During the onset and resolving phases of inflammation in murine peritonitis and bovine mastitis, Lf fragments of 15 and 17 kDa occurred in various body fluids, and the murine fragmentation, accumulation, and release were mediated initially by neutrophils and later by efferocytic macrophages. The 17-kDa fragment contained two bioactive tripeptides, FKD and FKE that promoted resolution phase macrophage conversion to a pro-resolving phenotype. This resulted in a reduction in peritoneal macrophage numbers and an increase in the CD11b(low) subset of these cells. Moreover, FKE, but not FKD, peptides enhanced efferocytosis of apoptotic PMN, reduced TNF alpha and interleukin (IL)-6, and increased IL-10 secretion by lipopolysaccharide-stimulated macrophages ex vivo. In addition, FKE promoted neutrophil-mediated resolution at high concentrations (100 mu M) by enhancing the formation of cytokine-scavenging aggregated NETs (tophi) at a low cellular density. Thus, PMN Lf is processed, acquired, and "recycled" by neutrophils and macrophages during inflammation resolution to generate fragments and peptides with paramount pro-resolving activities.
Authors with CRIS profile
Malgorzata Podolska
Department of Medicine 3 – Rheumatology and Immunology
Martin Herrmann
Department of Medicine 3 – Rheumatology and Immunology
Luis Munoz Becerra
Department of Medicine 3 – Rheumatology and Immunology
Christine Schauer
Department of Medicine 3 – Rheumatology and Immunology
Involved external institutions
Israel (IL)How to cite
APA:
Lutaty, A., Soboh, S., Schif-Zuck, S., Zeituni-Timor, O., Rostoker, R., Podolska, M.,... Schauer, C. (2018). A 17-kDa Fragment of Lactoferrin Associates With the Termination of Inflammation and Peptides Within Promote Resolution. Frontiers in Immunology, 9, 77-77. https://doi.org/10.3389/fimmu.2018.00644
MLA:
Lutaty, Aviv, et al. "A 17-kDa Fragment of Lactoferrin Associates With the Termination of Inflammation and Peptides Within Promote Resolution." Frontiers in Immunology 9 (2018): 77-77.
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