The impact of preanalytical variables on measuring cerebrospinal fluid biomarkers for Alzheimer's disease diagnosis: A review

Hansson O, Mikulskis A, Fagan AM, Teunissen C, Zetterberg H, Vanderstichele H, Molinuevo JL, Shaw LM, Vandijck M, Verbeek MM, Savage M, Mattsson N, Lewczuk P, Batrla R, Rutz S, Dean RA, Blennow K (2018)

Publication Type: Journal article

Publication year: 2018

Journal

Alzheimers & Dementia Elsevier Masson

Book Volume: 14

Pages Range: 1313-1333

Journal Issue: 10

DOI: 10.1016/j.jalz.2018.05.008

Abstract

INTRODUCTION: Cerebrospinal fluid (CSF) biomarkers have the potential to improve the diagnostic accuracy of Alzheimer's disease, yet there is a lack of harmonized preanalytical CSF handling protocols. METHODS: This systematic review summarizes the current literature on the influence of preanalytical variables on CSF biomarker concentration. We evaluated the evidence for three core CSF biomarkers: β-amyloid 42, total tau, and phosphorylated tau. RESULTS: The clinically important variables with the largest amount of conflicting data included the temperature at which samples are stored, the time nonfrozen samples can be stored, and possible effects of additives such as detergents, blood contamination, and centrifugation. Conversely, we discovered that there is consensus that tube material has a significant effect. DISCUSSION: A unified CSF handling protocol is recommended to reduce preanalytical variability and facilitate comparison of CSF biomarkers across studies and laboratories. In future, experiments should use a gold standard with fresh CSF collected in low binding tubes.

Authors with CRIS profile

Piotr Lewczuk Department of Psychiatry and Psychotherapy

Involved external institutions

Vrije Universiteit Amsterdam (VU) / University Amsterdam NL Netherlands (NL) Fujirebio Europe NV BE Belgium (BE) ADx NeuroSciences BE Belgium (BE) Skåne University Hospital / Skånes universitetssjukhus SE Sweden (SE) University of Pennsylvania US United States (USA) (US) Merck & Co., Inc. / Merck Sharp & Dohme Corp (MSD) US United States (USA) (US) Radboud University Nijmegen NL Netherlands (NL) Sahlgrenska University Hospital / Sahlgrenska Universitetssjukhuset SE Sweden (SE) Indiana University – Purdue University Indianapolis US United States (USA) (US) Roche Diagnostics GmbH DE Germany (DE) Washington University in St. Louis US United States (USA) (US) UK Dementia Research Institute (UK DRI) GB United Kingdom (GB) Pasqual Maragall Foundation ES Spain (ES)

How to cite

APA:

Hansson, O., Mikulskis, A., Fagan, A.M., Teunissen, C., Zetterberg, H., Vanderstichele, H.,... Blennow, K. (2018). The impact of preanalytical variables on measuring cerebrospinal fluid biomarkers for Alzheimer's disease diagnosis: A review. Alzheimers & Dementia, 14(10), 1313-1333. https://doi.org/10.1016/j.jalz.2018.05.008

MLA:

Hansson, Oskar, et al. "The impact of preanalytical variables on measuring cerebrospinal fluid biomarkers for Alzheimer's disease diagnosis: A review." Alzheimers & Dementia 14.10 (2018): 1313-1333.

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