Integrated Biosensor for Rapid and Point-of-Care Sepsis Diagnosis

Min J, Nothing MG, Coble B, Zheng H, Park J, Im H, Castro CM, Swirski FK, Weissleder R, Lee H, Weber G (2018)

Publication Type: Journal article

Publication year: 2018

Journal

ACS nano American Chemical Society

Book Volume: 12

Pages Range: 3378-3384

Journal Issue: 4

DOI: 10.1021/acsnano.7b08965

Abstract

Sepsis is an often fatal condition that arises when the immune response to an infection causes widespread systemic organ injury. A critical unmet need in combating sepsis is the lack of accurate early biomarkers that produce actionable results in busy clinical settings. Here, we report the development of a point-of-care platform for rapid sepsis detection. Termed IBS (integrated biosensor for sepsis), our approach leverages (i) the pathophysiological role of cytokine interleukin-3 (IL-3) in early sepsis and (ii) a hybrid magneto-electrochemical sensor for IL-3 detection. The developed platform produces test results within 1 h from native blood samples and detects IL-3 at a sensitivity of <10 pg/mL; this performance is >5-times faster and >10-times more sensitive than conventional enzyme-linked immunoadsorbent assays, the current gold standard. Using clinical samples, we show that elevated plasma IL-3 levels are associated with high organ failure rate and thus greater risk of mortality, confirming the potential of IL-3 as a sepsis diagnostic biomarker. With further system development ( e. g., full automation, data security measures) and rigorous validation studies, the compact and fast IBS could be a practical clinical tool for timely diagnosis and proactive treatment of sepsis.

Authors with CRIS profile

Maria Grazia Nothing Department of Surgery Georg Weber Department of Surgery

Involved external institutions

Massachusetts General Hospital US United States (USA) (US)

How to cite

APA:

Min, J., Nothing, M.G., Coble, B., Zheng, H., Park, J., Im, H.,... Weber, G. (2018). Integrated Biosensor for Rapid and Point-of-Care Sepsis Diagnosis. ACS nano, 12(4), 3378-3384. https://doi.org/10.1021/acsnano.7b08965

MLA:

Min, Jouha, et al. "Integrated Biosensor for Rapid and Point-of-Care Sepsis Diagnosis." ACS nano 12.4 (2018): 3378-3384.

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