Sample collection for laboratory-based study of the nasal airway and sinuses: a research compendium

Massey CJ, Del Valle FD, Abuzeid WM, Levy JM, Müller SK, Levine CG, Smith SS, Bleier BS, Ramakrishnan VR (2019)


Publication Type: Journal article

Publication year: 2019

Journal

DOI: 10.1002/alr.22510

Abstract

Background Collection of biologic samples from the nasal cavity and paranasal sinuses is of critical importance to the study of infectious or inflammatory conditions that affect both upper and lower airways. Numerous techniques for the study of ex-vivo samples exist, with specific applications, strengths, and weaknesses associated with each of them. In this compendium we summarize the available methods for collection of primary human samples and incorporate expert discussion of the pros, cons, and applications associated with each technique. Methods An expert panel containing members of the American Rhinologic Society's Research and Grants Committee compiled this educational reference. Rationale for use and the potential advantages and disadvantages are discussed. Research protocols and key references are enumerated. Results Sampling of the nasal cavity and paranasal sinuses can be achieved through a number of methods. Nonspecific sinonasal secretions may be collected via forced exhalation, nasal lavage, and nasal spray aspiration. Targeted collection of sinonasal secretions may be achieved via endoscopic placement of absorbent matrices. Nasal cytology or collection of superficial epithelium may be completed via brushing or scraping of endonasal structures. Collection of mucosal biopsies may be completed via sinonasal explant or full-thickness biopsy. Conclusion Multiple sampling techniques are available to collect biologic samples from the sinonasal cavity. These techniques differ in their ease of application, reproducibility, sample yield, and utility for different sinonasal pathologies or research goals. An appreciation of the benefits and drawbacks of each approach will allow investigators to select the techniques most appropriate for achieving research objectives.

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How to cite

APA:

Massey, C.J., Del Valle, F.D., Abuzeid, W.M., Levy, J.M., Müller, S.K., Levine, C.G.,... Ramakrishnan, V.R. (2019). Sample collection for laboratory-based study of the nasal airway and sinuses: a research compendium. International Forum of Allergy and Rhinology. https://doi.org/10.1002/alr.22510

MLA:

Massey, Conner J., et al. "Sample collection for laboratory-based study of the nasal airway and sinuses: a research compendium." International Forum of Allergy and Rhinology (2019).

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