Multi-scale imaging and analysis identify pan-embryo cell dynamics of germlayer formation in zebrafish

Shah G, Thierbach K, Schmid B, Waschke J, Reade A, Hlawitschka M, Roeder I, Scherf N, Huisken J (2019)

Publication Type: Journal article

Publication year: 2019

Journal

Nature Communications Nature Publishing Group: Nature Communications

Book Volume: 10

Article Number: 5753

Journal Issue: 1

DOI: 10.1038/s41467-019-13625-0

Abstract

The coordination of cell movements across spatio-temporal scales ensures precise positioning of organs during vertebrate gastrulation. Mechanisms governing such morphogenetic movements have been studied only within a local region, a single germlayer or in whole embryos without cell identity. Scale-bridging imaging and automated analysis of cell dynamics are needed for a deeper understanding of tissue formation during gastrulation. Here, we report pan-embryo analyses of formation and dynamics of all three germlayers simultaneously within a developing zebrafish embryo. We show that a distinct distribution of cells in each germlayer is established during early gastrulation via cell movement characteristics that are predominantly determined by their position in the embryo. The differences in initial germlayer distributions are subsequently amplified by a global movement, which organizes the organ precursors along the embryonic body axis, giving rise to the blueprint of organ formation. The tools and data are available as a resource for the community.

Authors with CRIS profile

Benjamin Schmid Optical Imaging Center Erlangen (OICE)

Involved external institutions

University of California San Francisco (UCSF) US United States (USA) (US) Max-Planck-Institut für molekulare Zellbiologie und Genetik / Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG) DE Germany (DE) Universitätsklinikum Carl Gustav Carus Dresden DE Germany (DE) Max-Planck-Institut für Kognitions- und Neurowissenschaften / Max Planck Institute for Human Cognitive and Brain Sciences DE Germany (DE) Leipzig University of Applied Sciences / Hochschule für Technik, Wirtschaft und Kultur Leipzig (HTWK) DE Germany (DE)

How to cite

APA:

Shah, G., Thierbach, K., Schmid, B., Waschke, J., Reade, A., Hlawitschka, M.,... Huisken, J. (2019). Multi-scale imaging and analysis identify pan-embryo cell dynamics of germlayer formation in zebrafish. Nature Communications, 10(1). https://doi.org/10.1038/s41467-019-13625-0

MLA:

Shah, Gopi, et al. "Multi-scale imaging and analysis identify pan-embryo cell dynamics of germlayer formation in zebrafish." Nature Communications 10.1 (2019).

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