Postnatal morphological lung development of wild type and CD26/DPP4 deficient rat pups in dependency of LPS exposure
Wagener I, Jungen M, von Hörsten S, Stephan M, Schmiedl A (2019)
Publication Type: Journal article
Publication year: 2019
Journal
Article Number: 151423
DOI: 10.1016/j.aanat.2019.151423
Abstract
Background: Rodents are born with morphological immature lungs and an intact surfactant system. CD26/DPP4 is a multifactorial transmembrane integral type II protein, which is involved in physiological and pathophysiological processes and is already expressed during development. CD26/DPP4, called CD26 in the following, is able to enhance or dampen differently triggered inflammation. LPS exposure often used to simulate perinatal infection delays lung development. Objective: A perinatal LPS rat model was used to test the hypothesis that CD26 deficiency modulates LPS-induced retardation in morphological lung development. Methods: New born Fischer CD26 positive (CD26+) and deficient (CD26−) rats were exposed to LPS on postnatal day (day post partum, dpp) 3 and 5. Morphological parameters of lung development were determined stereologically. Lung development was analysed in 7, 10 14 and 21day old rats. Results: Compared to controls LPS application resulted (1) in a mild inflammation independent of the strain, (2) in significantly lower total surface and volume of alveolar septa combined with significantly higher total volume of airspaces and alveolar size on dpp 7 in both substrains. However, compared to controls in LPS treated CD26− rats significant lower values of total septal surface and volume combined with higher values of total parenchymal airspaces and alveolar size were found until the end of classical alveolarization (dpp14). In LPS treated CD26+ rat pups the retardation was abolished already on dpp 10. Conclusion: In absence of CD26, LPS enhances the delay of morphological lung development. Morphological recovery was slower after the end of LPS exposure in CD26 deficient lungs.
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Germany (DE)How to cite
APA:
Wagener, I., Jungen, M., von Hörsten, S., Stephan, M., & Schmiedl, A. (2019). Postnatal morphological lung development of wild type and CD26/DPP4 deficient rat pups in dependency of LPS exposure. Annals of Anatomy-Anatomischer Anzeiger. https://doi.org/10.1016/j.aanat.2019.151423
MLA:
Wagener, Inga, et al. "Postnatal morphological lung development of wild type and CD26/DPP4 deficient rat pups in dependency of LPS exposure." Annals of Anatomy-Anatomischer Anzeiger (2019).
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