Tetramerization of human guanylate-binding protein 1 is mediated by coiled-coil formation of the C-terminal α-helices
Syguda A, Bauer M, Benscheid U, Ostler N, Naschberger E, Ince S, Stürzl M, Herrmann C (2012)
Publication Type: Journal article
Publication year: 2012
Journal
Book Volume: 279
Pages Range: 2544-2554
Journal Issue: 14
DOI: 10.1111/j.1742-4658.2012.08637.x
Abstract
The human guanylate-binding protein 1 (hGBP1) is a large GTP-binding protein belonging to the dynamin family, a common feature of which is nucleotide-dependent assembly to homotypic oligomers. Assembly leads to stimulation of GTPase activity, which, in the case of dynamin, is responsible for scission of vesicles from membranes. By yeast two-hybrid and biochemical experiments we addressed intermolecular interactions between all subdomains of hGBP1 and identified the C-terminal subdomain, α12/13, as a new interaction site for self-assembly. α12/13 represents a stable subdomain of hGBP1, as shown by CD spectroscopy. In addition to contacts between GTPase domains leading to dimer formation, the interaction between two α12/13 subdomains, in the course of GTP hydrolysis, results in tetramer formation of the protein. With the help of CD spectroscopy we showed coiled-coil formation of two α12/13 subdomains and concentration-dependent measurements allow estimating a value for the dissociation constant of 7.3 μm. We suggest GTP hydrolysis-driven release of the α12/13 subdomain, making it available for coiled-coil formation. Furthermore, we can demonstrate the biological relevance of hGBP1 tetramer formation in living cells by chemical cross-link experiments. © 2012 FEBS.
Authors with CRIS profile
Nicole Ostler
Professur für Molekulare und Experimentelle Chirurgie
Elisabeth Naschberger
Medizinische Fakultät
Michael Stürzl
Professur für Molekulare und Experimentelle Chirurgie
Involved external institutions
Germany (DE)How to cite
APA:
Syguda, A., Bauer, M., Benscheid, U., Ostler, N., Naschberger, E., Ince, S.,... Herrmann, C. (2012). Tetramerization of human guanylate-binding protein 1 is mediated by coiled-coil formation of the C-terminal α-helices. Febs Journal, 279(14), 2544-2554. https://doi.org/10.1111/j.1742-4658.2012.08637.x
MLA:
Syguda, Adrian, et al. "Tetramerization of human guanylate-binding protein 1 is mediated by coiled-coil formation of the C-terminal α-helices." Febs Journal 279.14 (2012): 2544-2554.
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