Visualizing cellular secretion with single-protein sensitivity via interferometric scattering microscopy (iSCAT)
Gemeinhardt A, König K, Nicoli F, Dahmardeh M, Sandoghdar V (2019)
Publication Type: Conference contribution
Publication year: 2019
Publisher: Institute of Electrical and Electronics Engineers Inc.
Conference Proceedings Title: 2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference, CLEO/Europe-EQEC 2019
Event location: Munich
ISBN: 9781728104690
DOI: 10.1109/CLEOE-EQEC.2019.8872994
Abstract
Proteins are involved in a large number of biological processes. Apart from the field of proteomics, where ensemble studies of the entire set of proteins present in a system are carried out, detection and analysis of single proteins has become a vibrant field of research. Particularly, the study of proteins that are secreted from cells into the extracellular space is an important topic with wide implications for basic intercellular interactions and immunology. Secretory proteins are responsible for a vast amount of cellular functions involving migration, wound healing, immunological response, or intercellular communication. Challenges in the traditional methods such as immunoassays, Western blotting, mass spectrometry, or fluorescence microscopy are the need for labeling (with immunological complements, isotopes, or fluorescent markers) and the necessary processing and separation steps that hinder high temporal resolution in dynamic studies. Today, none of the existing techniques is able to detect single proteins secreted from living cells at subsecond temporal resolution without the need for labeling.
Authors with CRIS profile
André Gemeinhardt
Lehrstuhl für Experimentalphysik
Katharina König
Lehrstuhl für Experimentalphysik
Vahid Sandoghdar
Lehrstuhl für Experimentalphysik
Involved external institutions
How to cite
APA:
Gemeinhardt, A., König, K., Nicoli, F., Dahmardeh, M., & Sandoghdar, V. (2019). Visualizing cellular secretion with single-protein sensitivity via interferometric scattering microscopy (iSCAT). In 2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference, CLEO/Europe-EQEC 2019. Munich, DE: Institute of Electrical and Electronics Engineers Inc..
MLA:
Gemeinhardt, André, et al. "Visualizing cellular secretion with single-protein sensitivity via interferometric scattering microscopy (iSCAT)." Proceedings of the 2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference, CLEO/Europe-EQEC 2019, Munich Institute of Electrical and Electronics Engineers Inc., 2019.
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