Characterization of dry eye disease in a mouse model by optical coherence tomography and fluorescein staining
Messner A, Fischak C, Pfister M, Schuetzenberger K, Garreis F, Paulsen F, Stegmann H, Dos Santos VA, Garhoefer G, Schmetterer L, Werkmeister RM (2019)
Publication Type: Journal article
Publication year: 2019
Journal
Book Volume: 10
Pages Range: 4884-4895
Journal Issue: 9
Abstract
A custom-built ultrahigh-resolution optical coherence tomography (UHR-OCT) system and fluorescein staining were employed tier investigation of a scopolamine induced dry eye mouse model. Acquired data was used to evaluate common and complementary findings of the two modalities. Central corneal thickness as measured by UHR-OCT increased significantly over the study period of 24 hours, from 89.0 +/- 3.57 mu m to 92.2 +/- 4.07 mu m. Both techniques were able to show corneal lesions with a large range of severity. Localized fluorescein staining was detected in 5% and diffuse staining in 45 % of cases where no epithelial damage was visible with OCT. However, OCT revealed stromal defects in 6% and endothelial defects in 18% of the cases, which could not be visualized via fluorescein staining. Thus, while fluorescein staining widely detected defects of the corneal surface in a mouse model of dry eye disease, OCT non-invasively revealed additional intimation about defect depth and involvement of particular layers. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
Authors with CRIS profile
Fabian Garreis
Lehrstuhl für Funktionelle und Klinische Anatomie
Friedrich Paulsen
Lehrstuhl für Funktionelle und Klinische Anatomie
Involved external institutions
Austria (AT)How to cite
APA:
Messner, A., Fischak, C., Pfister, M., Schuetzenberger, K., Garreis, F., Paulsen, F.,... Werkmeister, R.M. (2019). Characterization of dry eye disease in a mouse model by optical coherence tomography and fluorescein staining. Biomedical Optics Express, 10(9), 4884-4895. https://doi.org/10.1364/BOE.10.004884
MLA:
Messner, Alina, et al. "Characterization of dry eye disease in a mouse model by optical coherence tomography and fluorescein staining." Biomedical Optics Express 10.9 (2019): 4884-4895.
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