Wong DD, Van Zuylen WJ, Hamilton ST, Steingruber M, Sonntag E, Marschall M, Rawlinson WD (2019)
Publication Type: Journal article
Publication year: 2019
Book Volume: 63
Article Number: e02425-18
Journal Issue: 9
DOI: 10.1128/AAC.02425-18
Mutations in the cytomegalovirus UL97 kinase gene contribute to antiviral resistance. Mutations A594S and G598D from two clinical isolates were analyzed, and bacterial artificial chromosome (BAC)-engineered A594S recombinant cytomegalovirus exhibited a ganciclovir-resistant phenotype on plaque reduction. Viral replication was comparable to that of the wild type. Cell-based kinase activity and autophosphorylation of ectopically expressed proteins showed that mutants retained some kinase activity. This study showed that patient-derived cytomegalovirus with different ganciclovir sensitivities retained replication efficiency and exhibited some kinase activity in vitro.
APA:
Wong, D.D., Van Zuylen, W.J., Hamilton, S.T., Steingruber, M., Sonntag, E., Marschall, M., & Rawlinson, W.D. (2019). Patient-derived cytomegaloviruses with different ganciclovir sensitivities from UL97 mutation retain their replication efficiency and some kinase activity in vitro. Antimicrobial Agents and Chemotherapy, 63(9). https://doi.org/10.1128/AAC.02425-18
MLA:
Wong, Diana D., et al. "Patient-derived cytomegaloviruses with different ganciclovir sensitivities from UL97 mutation retain their replication efficiency and some kinase activity in vitro." Antimicrobial Agents and Chemotherapy 63.9 (2019).
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