Experimental Cutaneous Leishmaniasis: Mouse Models for Resolution of Inflammation Versus Chronicity of Disease

Bogdan C, Debus A, Sebald H, Rai B, Schäfer J, Obermeyer S, Schleicher U (2019)

Publication Type: Book chapter / Article in edited volumes

Publication year: 2019

Journal

Methods in Molecular Biology Humana Press

Publisher: Humana Press Inc.

Edited Volumes: Leishmania

Series: Methods in Molecular Biology

Book Volume: 1971

Pages Range: 315-349

DOI: 10.1007/978-1-4939-9210-2_18

Abstract

Experimental cutaneous leishmaniasis of mice is a valuable model to study the immune response to the protozoan pathogen Leishmania and to define mechanisms of parasite control and resolution of inflammation as well as of parasite evasion and chronicity of disease. In addition, over many years Leishmania-infected mice have been successfully used to analyze the function of newly discovered immune cell types, transcription factors, cytokines, and effector mechanisms in vivo. In this chapter we present detailed protocols for the culture, propagation, and inoculation of Leishmania promastigotes, the monitoring of the course of cutaneous infection, the determination of the tissue parasite burden and for the phenotyping of the ensuing immune response. The focus lies on the L. major mouse model, but an overview on other established models of murine cutaneous leishmaniasis is also provided.

Authors with CRIS profile

Christian Bogdan Lehrstuhl für Mikrobiologie und Infektionsimmunologie Baplu Rai Institute of Microbiology – Clinical Microbiology, Immunology and Hygiene Johanna Schäfer Institute of Microbiology – Clinical Microbiology, Immunology and Hygiene Stephanie Obermeyer Lehrstuhl für Mikrobiologie und Infektionsimmunologie Ulrike Schleicher Institute of Microbiology – Clinical Microbiology, Immunology and Hygiene

Additional Organisation(s)

Interdisziplinäres Zentrum für Klinische Forschung

How to cite

APA:

Bogdan, C., Debus, A., Sebald, H., Rai, B., Schäfer, J., Obermeyer, S., & Schleicher, U. (2019). Experimental Cutaneous Leishmaniasis: Mouse Models for Resolution of Inflammation Versus Chronicity of Disease. In Joachim Clos (Eds.), Leishmania. (pp. 315-349). Humana Press Inc..

MLA:

Bogdan, Christian, et al. "Experimental Cutaneous Leishmaniasis: Mouse Models for Resolution of Inflammation Versus Chronicity of Disease." Leishmania. Ed. Joachim Clos, Humana Press Inc., 2019. 315-349.

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