Reduction of X-ray-induced DNA damage in normal human cells treated with the PrC-210 radioprotector
Brand M, Sommer M, Jermusek F, Fahl WE, Uder M (2018)
Publication Type: Journal article
Publication year: 2018
Journal
Book Volume: 7
Journal Issue: 10
DOI: 10.1242/bio.035113
Abstract
The aim of our study was to determine the protective efficacy of the PrC-210 aminothiol radioprotector against X-ray-induced DNA damage in normal human cells and to establish dose- and time-effect models for future PrC-210 use in humans. The PrC-210 structure has a branched structure which enables scavenging of reactive oxygen species (ROS) away from DNA. Normal human blood lymphocytes, fibroblasts and naked genomic DNA were exposed to PrC-210 seconds to hours prior to irradiation. Biological (γ-H2AX foci), chemical (8-oxo-deoxyguanosine) and physical (genomic DNA electrophoretic migration) DNA damage endpoints were scored to determine the ability of PrC-210 to suppress radiation-induced DNA damage. X-ray-induced γ-H2AX foci in blood lymphocytes were reduced by 80% after irradiation with 10, 50 and 100 mGy, and DNA double-strand breaks in fibroblasts were reduced by 60% after irradiation with 20 Gy. Additionally, we observed a reduction of 8-oxo-deoxyguanosine (an ROS-mediated, DNA damage marker) in human genomic DNA to background in a PrC-210 dose-dependent manner. PrC-210 also eliminated radiation-induced cell death in colony formation assays after irradiation with 1 Gy. The protective efficacy of PrC-210 in each of these assay systems supports its development as a radioprotector for humans in multiple radiation exposure settings.
Authors with CRIS profile
Michael Brand
Lehrstuhl für Diagnostische Radiologie
Matthias Sommer
Institute of Radiology
Michael Uder
Lehrstuhl für Diagnostische Radiologie
Involved external institutions
United States (USA) (US)How to cite
APA:
Brand, M., Sommer, M., Jermusek, F., Fahl, W.E., & Uder, M. (2018). Reduction of X-ray-induced DNA damage in normal human cells treated with the PrC-210 radioprotector. Biology Open, 7(10). https://doi.org/10.1242/bio.035113
MLA:
Brand, Michael, et al. "Reduction of X-ray-induced DNA damage in normal human cells treated with the PrC-210 radioprotector." Biology Open 7.10 (2018).
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