Different Patterns of Cartilage Mineralization Analyzed by Comparison of Human, Porcine, and Bovine Laryngeal Cartilages

Claassen H, Schicht M, Fleiner B, Hillmann R, Hoogeboom S, Tillmann B, Paulsen F (2017)

Publication Type: Journal article

Publication year: 2017

Journal

Journal of Histochemistry & Cytochemistry Histochemical Society / SAGE Publications (UK and US)

Book Volume: 65

Pages Range: 367-379

Journal Issue: 6

DOI: 10.1369/0022155417703025

Abstract

Laryngeal cartilages undergo a slow ossification process during aging, making them an excellent model for studying cartilage mineralization and ossification processes. Pig laryngeal cartilages are similar to their human counterparts in shape and size, also undergo mineralization, facilitating the study of cartilage mineralization. We investigated the processes of cartilage mineralization and ossification and compared these with the known processes in growth plates. Thyroid cartilages from glutaraldehyde-perfused male minipigs and from domestic pigs were used for X-ray, light microscopic, and transmission electron microscopic analyses. We applied different fixation and postfixation solutions to preserve cell shape, proteoglycans, and membranes. In contrast to the ossifying human thyroid cartilage, predominantly cartilage mineralization was observed in minipig and domestic pig thyroid cartilages. The same subset of chondrocytes responsible for growth plate mineralization is also present in thyroid cartilage mineralization. Besides mineralization mediated by matrix vesicles, a second pattern of cartilage mineralization was observed in thyroid cartilage only. Here, the formation and growth of crystals were closely related to collagen fibrils, which served as guide rails for the expansion of mineralization. It is hypothesized that the second pattern of cartilage mineralization may be similar to a maturation of mineralized cartilage after initial matrix vesicles-mediated cartilage mineralization.

Authors with CRIS profile

Martin Schicht Lehrstuhl für Funktionelle und Klinische Anatomie Friedrich Paulsen Lehrstuhl für Funktionelle und Klinische Anatomie

Involved external institutions

Martin-Luther-Universität Halle-Wittenberg (MLU) DE Germany (DE) Christian-Albrechts-Universität zu Kiel DE Germany (DE)

How to cite

APA:

Claassen, H., Schicht, M., Fleiner, B., Hillmann, R., Hoogeboom, S., Tillmann, B., & Paulsen, F. (2017). Different Patterns of Cartilage Mineralization Analyzed by Comparison of Human, Porcine, and Bovine Laryngeal Cartilages. Journal of Histochemistry & Cytochemistry, 65(6), 367-379. https://doi.org/10.1369/0022155417703025

MLA:

Claassen, Horst, et al. "Different Patterns of Cartilage Mineralization Analyzed by Comparison of Human, Porcine, and Bovine Laryngeal Cartilages." Journal of Histochemistry & Cytochemistry 65.6 (2017): 367-379.

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