1,25-dihydroxyvitamin D3 inhibits corneal wound healing in an ex-vivo mouse model

Sel S, Trau S, Paulsen F, Kalinski T, Stangl GI, Nass N (2016)

Publication Type: Journal article

Publication year: 2016

Journal

Graefes Archive For Clinical and Experimental Ophthalmology Springer Verlag (Germany)

Book Volume: 254

Pages Range: 717-24

Journal Issue: 4

DOI: 10.1007/s00417-016-3267-4

Abstract

PURPOSE: Impaired healing of corneal injuries can result in ulceration and complete loss of vision, especially in the elderly. Such patients frequently also exhibit vitamin D insufficiency. 1,25-dihydroxyvitamin D3 is the active vitamin D metabolite. As it affects cell proliferation and inflammation, we herein aimed at elucidating its influence on corneal wound healing after alkali burn by using in vitro and ex vivo techniques. METHODS: mRNA abundance in human corneal epithelial cells in response to vitamin D3 was determined by RT-PCR. Corneal re-epithelialization after alkaline burn was analyzed using enucleated mouse eyes and fluorescein staining. RESULTS: Human corneal epithelial cells (HCEC) expressed the vitamin D receptor (VDR) and retinoid x receptor (RXR) and were responsive to 1,25- dihydroxyvitamin D3, as shown by induction of the 1,25- dihydroxyvitamin D3 responsive gene cyp-24A1 and slightly reduced abundance of IL-6 mRNA. However, no effect on cell vitality and migration was observed. In contrast, re-epithelialization of mouse corneas ex vivo was dose dependently inhibited by 1,25- dihydroxyvitamin D3. CONCLUSIONS: These data indicate that topically applied 1,25- dihydroxyvitamin D3 does not seem to be suitable for therapy of corneal lesions.

Authors with CRIS profile

Friedrich Paulsen Lehrstuhl für Funktionelle und Klinische Anatomie

Involved external institutions

Ruprecht-Karls-Universität Heidelberg DE Germany (DE) Martin-Luther-Universität Halle-Wittenberg (MLU) DE Germany (DE) Otto-von-Guericke-Universität Magdeburg DE Germany (DE)

How to cite

APA:

Sel, S., Trau, S., Paulsen, F., Kalinski, T., Stangl, G.I., & Nass, N. (2016). 1,25-dihydroxyvitamin D3 inhibits corneal wound healing in an ex-vivo mouse model. Graefes Archive For Clinical and Experimental Ophthalmology, 254(4), 717-24. https://doi.org/10.1007/s00417-016-3267-4

MLA:

Sel, Saadettin, et al. "1,25-dihydroxyvitamin D3 inhibits corneal wound healing in an ex-vivo mouse model." Graefes Archive For Clinical and Experimental Ophthalmology 254.4 (2016): 717-24.

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