Heider S, Strobel J, Mempel W, Eckstein R, Weisbach VG (2016)
Publication Type: Journal article
Publication year: 2016
Book Volume: 62
Pages Range: 1857-1878
Journal Issue: 10
DOI: 10.7754/Clin.Lab.2016.160106
BACKGROUND: We present a multivariate test system using flow cytometry after in-vitro lymphocyte stimulation using 5 mitogens and 7 antigens to describe in-vitro immunofunction. METHODS: The present work is a crucial step towards establishing a simple, CFSE-based, multivariate test system that can describe the dynamics of stimulus-induced lymphocyte proliferation with considerably more precision than is possible with the radionucleotide method using 3H-thymidine. Using multicolour flow cytometry, our method allows additional phenotyping of the proliferating cells and quantifies the proliferation behaviour by precisely resolving daughter generations and determining the precursor frequency. CONCLUSIONS: Taking the calculated apoptosis parameters into account not only provides additional information about the stimulus-specific response behaviour but also improves the validity of the commonly used proliferation indices. Not only can we confirm previous findings that healthy people have marked differences in a multivariate test system in terms of the individual in-vitro reactivity to various stimuli but also substantiate that the response pattern of an individual is remarkably constant. In follow-up studies we can show for the first time that the results of immunofunctional testing do not change over a period of at least 6 months and appear to be an inherent characteristic of the individual and thus possibly have a genetic basis.
APA:
Heider, S., Strobel, J., Mempel, W., Eckstein, R., & Weisbach, V.G. (2016). Measuring Lymphocyte Proliferation in Response to Specific Antigen and Mitogen Stimuli Using Flow Cytometry. Clinical Laboratory, 62(10), 1857-1878. https://doi.org/10.7754/Clin.Lab.2016.160106
MLA:
Heider, Stefan, et al. "Measuring Lymphocyte Proliferation in Response to Specific Antigen and Mitogen Stimuli Using Flow Cytometry." Clinical Laboratory 62.10 (2016): 1857-1878.
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