Decreased IL-10(+) regulatory B cells (Bregs) in lupus nephritis patients
Heinemann K, Wilde B, Hoerning A, Tebbe B, Kribben A, Witzke O, Dolff S (2016)
Publication Type: Journal article
Publication year: 2016
Journal
Book Volume: 45
Pages Range: 312-6
Journal Issue: 4
DOI: 10.3109/03009742.2015.1126346
Abstract
OBJECTIVES: Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by B cell-dependent autoantibody production. Recently, a new B-cell subset was discovered that has a regulatory capacity. The aim of this study was to analyse regulatory B cells (Bregs) in SLE patients. METHOD: Peripheral mononuclear blood cells (PBMCs) of 34 SLE patients fulfilling the American College of Rheumatology (ACR) criteria for SLE and 21 healthy controls (HC) were included. PBMCs were stained for CD19, CD24, and CD38 and analysed by flow cytometry. In vitro stimulated PBMCs with CpG and restimulated with phorbol 12-myristate 13-acetate (PMA) and ionomycin were investigated for IL-10(+) Bregs . RESULTS: The percentages of circulating CD19(+)CD24(hi)CD38(hi) cells in HC were not different those in from SLE patients. The percentages of IL-10(+) Bregs were significantly decreased in SLE patients, in particular those with lupus nephritis (LN), compared to HC. The proportion was independent of disease activity. CONCLUSIONS: This is the first study to demonstrate a decrease in IL-10-producing B cells in LN patients compared to HC, reflecting an impaired regulatory function.
Involved external institutions
Germany (DE)How to cite
APA:
Heinemann, K., Wilde, B., Hoerning, A., Tebbe, B., Kribben, A., Witzke, O., & Dolff, S. (2016). Decreased IL-10(+) regulatory B cells (Bregs) in lupus nephritis patients. Scandinavian Journal of Rheumatology, 45(4), 312-6. https://dx.doi.org/10.3109/03009742.2015.1126346
MLA:
Heinemann, K., et al. "Decreased IL-10(+) regulatory B cells (Bregs) in lupus nephritis patients." Scandinavian Journal of Rheumatology 45.4 (2016): 312-6.
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