Nanoparticle-based B-cell targeting vaccines: Tailoring of humoral immune responses by functionalization with different TLR-ligands

Zilker C, Kozlova D, Sokolova V, Yan H, Epple M, Überla K, Temchura V (2017)

Publication Type: Journal article

Publication year: 2017

Journal

Nanomedicine-Nanotechnology Biology and Medicine Elsevier

Book Volume: 13

Pages Range: 173-182

Journal Issue: 1

DOI: 10.1016/j.nano.2016.08.028

Abstract

Induction of an appropriate type of humoral immune response during vaccination is essential for protection against viral and bacterial infections. We recently observed that biodegradable calcium phosphate (CaP) nanoparticles coated with proteins efficiently targeted and activated naïve antigen-specific B-cells in vitro. We now compared different administration routes for CaP-nanoparticles and demonstrated that intramuscular immunization with such CaP-nanoparticles induced stronger immune responses than immunization with monovalent antigen. Additional functionalization of the CaP-nanoparticles with TRL-ligands allowed modulating the IgG subtype response and the level of mucosal IgA antibodies. CpG-containing CaP-nanoparticles were as immunogenic as a virus-like particle vaccine. Functionalization of CaP-nanoparticles with T-helper cell epitopes or CpG also allowed overcoming lack of T-cell help. Thus, our results indicate that CaP-nanoparticle-based B-cell targeting vaccines functionalized with TLR-ligands can serve as a versatile platform for efficient induction and modulation of humoral immune responses in vivo.

Authors with CRIS profile

Klaus Überla Lehrstuhl für Klinische und Molekulare Virologie

Involved external institutions

Chinese Academy of Sciences (CAS) / 中国科学院 CN China (CN) Ruhr-Universität Bochum (RUB) DE Germany (DE) Universität Duisburg-Essen (UDE) DE Germany (DE)

How to cite

APA:

Zilker, C., Kozlova, D., Sokolova, V., Yan, H., Epple, M., Überla, K., & Temchura, V. (2017). Nanoparticle-based B-cell targeting vaccines: Tailoring of humoral immune responses by functionalization with different TLR-ligands. Nanomedicine-Nanotechnology Biology and Medicine, 13(1), 173-182. https://doi.org/10.1016/j.nano.2016.08.028

MLA:

Zilker, Claudia, et al. "Nanoparticle-based B-cell targeting vaccines: Tailoring of humoral immune responses by functionalization with different TLR-ligands." Nanomedicine-Nanotechnology Biology and Medicine 13.1 (2017): 173-182.

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