Quantification of plaque lipids in the aortic root of ApoE-deficient mice by 3D DIXON magnetic resonance imaging in an ex vivo model

Dietel B, Budinsky L, Kühn C, Uder M, Achenbach S, Heß A (2015)

Publication Type: Journal article

Publication year: 2015

Journal

European Radiology Springer Verlag (Germany)

Book Volume: 25

Pages Range: 736-44

Journal Issue: 3

DOI: 10.1007/s00330-014-3456-7

Abstract

To establish a dedicated protocol for the three-dimensional (3D) quantification of plaque lipids in apolipoprotein E-deficient (apoE(-/-)) mice using ex vivo MRI.ApoE(-/-) mice were fed a high-fat diet (n = 10) or normal food (n = 10) for 3 months. Subsequently, a 3D FLASH MRI sequence was used to view the anatomy of the aortic root in the isolated hearts, where a 3D double-echo two-excitation pulse sequence (DIXON sequence) was used to selectively image plaque lipids. The vessel wall, lumen and plaque lipid volumes were quantified by MRI and histology for correlation analysis.DIXON MRI allowed visualisation and accurate quantification of plaque lipids. When comparing the vessel wall, lumen and plaque lipid sizes in the aortic root, Bland-Altman and linear regression analysis revealed a close correlation between MRI results and the histological data both on a slice-by-slice basis and of the volumetric measurements (vessel wall: r (2) = 0.775, p < 0.001; vessel lumen: r (2) = 0.875; p = 0.002; plaque lipid: r (2) = 0.819, p = 0.003).The combination of 3D FLASH and DIXON-sequence MRI permits an accurate ex vivo assessment of the investigated plaque parameters in the aortic root of mice, particularly the lipid content.o Ex vivo MRI allows high resolution quantification of plaque parameters in mice o DIXON MRI enables visualisation of plaque lipids in the aortic root of mice o A close correlation between histology and MRI quantifies plaque parameters o Preservation of tissue integrity allows additional analysis to be performed o Short measurement time may permit the translation into in vivo measurements.

Authors with CRIS profile

Barbara Dietel Graduiertenzentrum der FAU Constanze Kühn Department of Medicine 2 – Cardiology and Angiology Michael Uder Lehrstuhl für Diagnostische Radiologie Stephan Achenbach Department of Medicine 2 – Cardiology and Angiology Andreas Heß Lehrstuhl für Pharmakologie und Toxikologie

Involved external institutions

Vienna Biocenter VBC AT Austria (AT)

How to cite

APA:

Dietel, B., Budinsky, L., Kühn, C., Uder, M., Achenbach, S., & Heß, A. (2015). Quantification of plaque lipids in the aortic root of ApoE-deficient mice by 3D DIXON magnetic resonance imaging in an ex vivo model. European Radiology, 25(3), 736-44. https://doi.org/10.1007/s00330-014-3456-7

MLA:

Dietel, Barbara, et al. "Quantification of plaque lipids in the aortic root of ApoE-deficient mice by 3D DIXON magnetic resonance imaging in an ex vivo model." European Radiology 25.3 (2015): 736-44.

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