Piwi-like 1 and 4 gene transcript levels are associated with clinicopathological parameters in renal cell carcinomas
Al-Janabi O, Wach S, Nolte E, Weigelt K, Rau T, Stoehr C, Legal W, Schick S, Greither T, Hartmann A, Wullich B, Taubert H (2014)
Publication Type: Journal article
Publication year: 2014
Journal
Book Volume: 1842
Pages Range: 686-90
Journal Issue: 5
DOI: 10.1016/j.bbadis.2014.01.014
Abstract
Piwi-like gene family members (Piwil 1-4) are considered stem cell-associated genes/proteins. These are expressed predominantly in germline cells, but are re-expressed in different tumors. Piwil 1-4 gene expression has not previously been studied and correlated with clinicopathological parameters in renal cell carcinomas (RCC). The Piwil 1-4 transcript levels were analyzed by quantitative real-time PCR in 73 clear cell RCC (ccRCC) tissues and corresponding normal tissues. The transcript levels of Piwil 1, 2 and 4 were strongly and significantly correlated with each other, in both the tumor tissues and the normal tissues (P<0.001; Spearman's rank test). Piwil 4 gene expression was significantly higher in the ccRCC tissues than that in the corresponding normal renal tissues (P<0.001; Wilcoxon signed-rank test). When the ccRCC patient cohort was divided according to the median Piwil 1-4 expression into low- and high-expression groups and according to age into younger (<=64years) and older patient groups (>64years), the younger patients displayed significantly higher levels of Piwil 1 mRNA in comparison to the older patients (P=0.010; Fisher's exact test). Interestingly, Piwil 1 expression was left-right polarized in the normal tissues but not in the tumor tissues (P=0.004; Fisher's exact test). Altogether, associations were determined between the Piwi-like family member expression levels and clinicopathological parameters of ccRCC, suggesting a potential role for these genes/proteins in ccRCC diagnostics and tumorigenesis as well as in renal tissue embryology.
Authors with CRIS profile
Arndt Hartmann
Lehrstuhl für Allgemeine Pathologie und Pathologische Anatomie
Bernd Wullich
Lehrstuhl für Urologie
Helge Taubert
Urologische Klinik
Involved external institutions
Germany (DE)How to cite
APA:
Al-Janabi, O., Wach, S., Nolte, E., Weigelt, K., Rau, T., Stoehr, C.,... Taubert, H. (2014). Piwi-like 1 and 4 gene transcript levels are associated with clinicopathological parameters in renal cell carcinomas. Biochimica et Biophysica Acta - Molecular Basis of Disease, 1842(5), 686-90. https://doi.org/10.1016/j.bbadis.2014.01.014
MLA:
Al-Janabi, Omar, et al. "Piwi-like 1 and 4 gene transcript levels are associated with clinicopathological parameters in renal cell carcinomas." Biochimica et Biophysica Acta - Molecular Basis of Disease 1842.5 (2014): 686-90.
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