Influenza A virus drift variants reduced the detection sensitivity of a commercial multiplex nucleic acid amplification assay in the season 2014/15
Huzly D, Korn K, Bierbaum S, Eberle B, Falcone V, Knöll A, Steininger P, Panning M (2016)
Publication Type: Journal article
Publication year: 2016
Journal
Book Volume: 161
Pages Range: 2417-23
Journal Issue: 9
DOI: 10.1007/s00705-016-2930-8
Abstract
The influenza season 2014/15 was dominated by drift variants of influenza A(H3N2), which resulted in a reduced vaccine effectiveness. It was not clear if the performance of commercial nucleic-acid-based amplification (NAT) assays for the detection of influenza was affected. The purpose of this study was to perform a real-life evaluation of two commercial NAT assays. During January-April 2015, we tested a total of 665 samples from patients with influenza-like illness using the Fast Track Diagnostics Respiratory pathogens 21, a commercial multiplex kit, (cohorts 1 and 2, n = 563 patients) and the Xpert Flu/RSV XC assay (cohort 3, n = 102 patients), a single-use cartridge system. An in-house influenza real-time RT-PCR (cohort 1) and the RealStar Influenza RT-PCR 1.0 Kit (cohort 2 and 3) served as reference tests. Compared to the reference assay, an overall agreement of 95.9 % (cohort 1), 95 % (cohort 2), and 98 % (cohort 3) was achieved. A total of 24 false-negative results were observed using the Fast Track Diagnostics Respiratory pathogens 21 kit. No false-negative results occurred using the Xpert Flu/RSV XC assay. The Fast Track Diagnostics Respiratory pathogens 21 kit and the Xpert Flu/RSV XC assay had sensitivities of 90.7 % and 100 % and specificities of 100 % and 94.1 %, respectively, compared to the RealStar 1.0 kit. Upon modification of the Fast Track Diagnostics Respiratory pathogens 21 kit, the sensitivity increased to 97.3 %. Influenza virus strains circulating during the 2014/15 season reduced the detection sensitivity of a commercial NAT assay, and continuous monitoring of test performance is therefore necessary.
Authors with CRIS profile
Antje Knöll
Institute of Clinical and Molecular Virology
Philipp Steininger
Institute of Clinical and Molecular Virology
Involved external institutions
Germany (DE)How to cite
APA:
Huzly, D., Korn, K., Bierbaum, S., Eberle, B., Falcone, V., Knöll, A.,... Panning, M. (2016). Influenza A virus drift variants reduced the detection sensitivity of a commercial multiplex nucleic acid amplification assay in the season 2014/15. Archives of Virology, 161(9), 2417-23. https://doi.org/10.1007/s00705-016-2930-8
MLA:
Huzly, Daniela, et al. "Influenza A virus drift variants reduced the detection sensitivity of a commercial multiplex nucleic acid amplification assay in the season 2014/15." Archives of Virology 161.9 (2016): 2417-23.
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