The adaptor protein Swiprosin-1/EFhd2 is dispensable for platelet function in mice

Morowski M, Brachs S, Mielenz D, Nieswandt B, Duetting S (2014)

Publication Type: Journal article

Publication year: 2014

Journal

PLoS ONE Public Library of Science

Book Volume: 9

Pages Range: e107139

Journal Issue: 9

DOI: 10.1371/journal.pone.0107139

Abstract

Platelets are anuclear cell fragments derived from bone marrow megakaryocytes that safeguard vascular integrity, but may also cause pathological vessel occlusion. Reorganizations of the platelet cytoskeleton and agonist-induced intracellular Ca2+-mobilization are crucial for platelet hemostatic function. EF-hand domain containing 2 (EFhd2, Swiprosin-1) is a Ca2+-binding cytoskeletal adaptor protein involved in actin remodeling in different cell types, but its function in platelets is unknown.Based on the described functions of EFhd2 in immune cells, we tested the hypothesis that EFhd2 is a crucial adaptor protein for platelet function acting as a regulator of Ca2+-mobilization and cytoskeletal rearrangements.We generated EFhd2-deficient mice and analyzed their platelets in vitro and in vivo. Efhd2-/- mice displayed normal platelet count and size, exhibited an unaltered in vivo life span and showed normal Ca2+-mobilization and activation/aggregation responses to classic agonists. Interestingly, upon stimulation of the immunoreceptor tyrosine-based activation motif-coupled receptor glycoprotein (GP) VI, Efhd2-/- platelets showed a slightly increased coagulant activity. Furthermore, absence of EFhd2 had no significant impact on integrin-mediated clot retraction, actomyosin rearrangements and spreading of activated platelets on fibrinogen. In vivo EFhd2-deficiency resulted in unaltered hemostatic function and unaffected arterial thrombus formation.These results show that EFhd2 is not essential for platelet function in mice indicating that other cytoskeletal adaptors may functionally compensate its loss.

Authors with CRIS profile

Sebastian Brachs Department of Molecular Immunology Dirk Mielenz Medizinische Fakultät

Involved external institutions

Julius-Maximilians-Universität Würzburg DE Germany (DE)

How to cite

APA:

Morowski, M., Brachs, S., Mielenz, D., Nieswandt, B., & Duetting, S. (2014). The adaptor protein Swiprosin-1/EFhd2 is dispensable for platelet function in mice. PLoS ONE, 9(9), e107139. https://doi.org/10.1371/journal.pone.0107139

MLA:

Morowski, Martina, et al. "The adaptor protein Swiprosin-1/EFhd2 is dispensable for platelet function in mice." PLoS ONE 9.9 (2014): e107139.

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