Dimitrijevic M, Aleksic I, Vujic V, Stanojevic S, Pilipovic I, von Hörsten S, Leposavic G (2014)
Publication Type: Journal article
Publication year: 2014
Publisher: Springer Verlag (Germany)
Book Volume: 36
Pages Range: 9696
Journal Issue: 4
DOI: 10.1007/s11357-014-9696-2
In humans, usual aging, differently from successful aging, is associated with deregulation of proinflammatory/anti-inflammatory cytokine balance. The corresponding data from rat studies are limited. Therefore, we examined (i) cytokine messenger RNA (mRNA) profile of fresh peritoneal cells from 6- (adult), 24- (old), and 31-month-old (long-lived) AO rats and (ii) proinflammatory (IL-1? and IL-6) and anti-inflammatory (IL-10) cytokine, NO, and urea production in their LPS-stimulated cultures. Comparing with adult rats, cells from old ones expressed lower amount of TNF-? and IL-6 mRNAs, but greater amount of IL-1? mRNA. On the other hand, cells from long-lived rats exhibited a dramatic increase in IL-10 mRNA expression followed by diminished TNF-? and IL-6 mRNA expression, and comparable expression of IL-1? mRNA relative to adult rats. Consequently, IL-10/IL-1? mRNA ratio was greater in cells from long-lived rats than in adult and old rats. In LPS-stimulated peritoneal cell cultures (contained >=95 % macrophages) from old rats, concentration of common proinflammatory cytokines was higher than in those from adult rats. Comparing with adult and old rats, in LPS-stimulated macrophage cultures from long-lived rats, TNF-? and IL-6 concentrations were lower; IL-1? concentration was comparable or greater (in respect to adult rats), whereas that of IL-10 was strikingly higher. Consistently, in macrophage cultures from long-lived rats, NO (iNOS activity marker)/urea (arginase activity marker) ratio was less and not different from that in old and adult rats, respectively. The study suggests that macrophages from long-lived rats, differently from those of old ones, have substantial ability to limit proinflammatory mediator production, which may contribute to their longevity.
APA:
Dimitrijevic, M., Aleksic, I., Vujic, V., Stanojevic, S., Pilipovic, I., von Hörsten, S., & Leposavic, G. (2014). Peritoneal exudate cells from long-lived rats exhibit increased IL-10/IL-1? expression ratio and preserved NO/urea ratio following LPS-stimulation in vitro. Age, 36(4), 9696. https://doi.org/10.1007/s11357-014-9696-2
MLA:
Dimitrijevic, Mirjana, et al. "Peritoneal exudate cells from long-lived rats exhibit increased IL-10/IL-1? expression ratio and preserved NO/urea ratio following LPS-stimulation in vitro." Age 36.4 (2014): 9696.
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