Low dose ionising radiation leads to a NF-?B dependent decreased secretion of active IL-1? by activated macrophages with a discontinuous dose-dependency

Lödermann B, Wunderlich R, Frey S, Schorn C, Stangl S, Roedel F, Keilholz L, Fietkau R, Gaipl U, Frey B (2012)

Publication Type: Journal article

Publication year: 2012

Journal

International Journal of Radiation Biology Informa Healthcare

Book Volume: 88

Pages Range: 727-34

Journal Issue: 10

DOI: 10.3109/09553002.2012.689464

Abstract

Therapy with low doses of ionising radiation (X-rays) exerts anti-inflammatory effects. Little is known about whether and how low doses of X-ray treatment modulate the inflammatory phenotype of macrophages, especially the secretion of Interleukin-1beta (IL-1?).Macrophages were differentiated from human THP-1 monocytes, activated with lipopolysaccharide (LPS), treated with distinct low doses of X-rays, and co-activated with monosodium urate crystals (MSU) to induce inflammasome activation. Secretion of IL-1? was analysed by an enzyme-linked immunosorbent assay (ELISA) and Western blot. Furthermore, we analysed the intracellular amounts of the serine/threonine protein kinase B (named: Akt), mitogen-activated protein kinase p38 (p38), the v-rel reticuloendotheliosis viral oncogene homolog A (RelA), and pro- and cleaved IL-1?.Low dose X-rays led to decreased secretion of active IL-1? in a manner discontinuous with dose which was most pronounced after 0.5 or 0.7 Gy. Passive release of lactate dehydrogenase (LDH) was not influenced by X-rays. The decreased secretion of IL-1? correlated with reduced translocation of RelA, being part of the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-?B) complex, into the nucleus. After 0.5 or 0.7 Gy of X-rays, the intracellular protein amounts of up (p38) and downstream molecules (Akt) of NF-?B were reduced in activated macrophages, as were the pro- and cleaved forms of IL-1?.Distinct low doses of X-rays induce an anti-inflammatory phenotype of activated macrophages by lowering the amount of secreted IL-1? in a NF-?B dependent manner.

Authors with CRIS profile

Barbara Lödermann Medizinische Fakultät Roland Wunderlich Department of Radiation Oncology Silke Frey Department of Medicine 3 – Rheumatology and Immunology Rainer Fietkau Lehrstuhl für Strahlentherapie Udo Gaipl Department of Radiation Oncology Benjamin Frey Department of Radiation Oncology

Involved external institutions

Goethe-Universität Frankfurt am Main DE Germany (DE) Klinikum Bayreuth DE Germany (DE)

How to cite

APA:

Lödermann, B., Wunderlich, R., Frey, S., Schorn, C., Stangl, S., Roedel, F.,... Frey, B. (2012). Low dose ionising radiation leads to a NF-?B dependent decreased secretion of active IL-1? by activated macrophages with a discontinuous dose-dependency. International Journal of Radiation Biology, 88(10), 727-34. https://doi.org/10.3109/09553002.2012.689464

MLA:

Lödermann, Barbara, et al. "Low dose ionising radiation leads to a NF-?B dependent decreased secretion of active IL-1? by activated macrophages with a discontinuous dose-dependency." International Journal of Radiation Biology 88.10 (2012): 727-34.

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