Sonnewald U (2001)
Publication Status: Published
Publication Type: Journal article
Publication year: 2001
Publisher: KLUWER ACADEMIC PUBL
Book Volume: 7
Pages Range: 221-227
Journal Issue: 3
A novel selection marker for plant transformation alternative to antibiotic and herbicide resistance is described. The selective agent applied is 2-deoxyglucose (2-DOG) which in the cytosol of plant cells is phosphorylated by hexokinase yielding 2-DOG-6-phosphate (2-DOG-6-P). 2-DOG-6-P exerts toxic effects on overall cellular metabolism leading to cell death. We observed that constitutive expression of the yeast DOG(R) 1 gene encoding a 2-DOG-6-P phosphatase resulted in resistance towards 2-DOG in transgenic tobacco plants. This finding was exploited to develop a selection system during transformation of tobacco and potato plants. The lowest concentration of 2-DOG leading to nearly complete inhibition of regeneration of wild-type explants was found to range between 400 and 600 mg/12-DOG for tobacco. potato and tomato plants. After Agrobacterium tumefaciens-mediated transformation cells expressing the DOG(R) 1 gene were selected by resistance to 2-DOG. More than 50% of tobacco explants formed shoots and on average 50% of these shoots harboured the DOG(R) 1 gene. Similar results were obtained for potato cv. Solara. The acceptability of the resistance gene derived from baker's yeast, the unobjectionable toxicological data of 2-DOG as well as the normal phenotype of DOG(R) 1-expressing plants support the use of this selection system in crop plant transformation.
APA:
Sonnewald, U. (2001). 2-Deoxyglucose resistance: a novel selection marker for plant transformation. Molecular Breeding, 7(3), 221-227.
MLA:
Sonnewald, Uwe. "2-Deoxyglucose resistance: a novel selection marker for plant transformation." Molecular Breeding 7.3 (2001): 221-227.
BibTeX: Download