miR-638 promotes melanoma metastasis and protects melanoma cells from apoptosis and autophagy

Bhattacharya A, Schmitz U, Raatz Y, Schoenherr M, Kottek T, Schauer M, Franz S, Saalbach A, Anderegg U, Wolkenhauer O, Schadendorf D, Simon JC, Magin T, Vera González J, Kunz M (2015)

Publication Type: Journal article

Publication year: 2015

Journal

Oncotarget Impact Journals

Book Volume: 6

Pages Range: 2966-80

Journal Issue: 5

DOI: 10.18632/oncotarget.3070

Abstract

The present study identified miR-638 as one of the most significantly overexpressed miRNAs in metastatic lesions of melanomas compared with primary melanomas. miR-638 enhanced the tumorigenic properties of melanoma cells in vitro and lung colonization in vivo. mRNA expression profiling identified new candidate genes including TP53INP2 as miR-638 targets, the majority of which are involved in p53 signalling. Overexpression of TP53INP2 severely attenuated proliferative and invasive capacity of melanoma cells which was reversed by miR-638. Depletion of miR-638 stimulated expression of p53 and p53 downstream target genes and induced apoptosis and autophagy. miR-638 promoter analysis identified the miR-638 target transcription factor associated protein 2? (TFAP2A/AP-2?) as a direct negative regulator of miR-638, suggestive for a double-negative regulatory feedback loop. Taken together, miR-638 supports melanoma progression and suppresses p53-mediated apoptosis pathways, autophagy and expression of the transcriptional repressor TFAP2A/AP-2?.

Authors with CRIS profile

Julio Vera González Professur für Tumorimmunologie (Schwerpunkt Systembiologie)

Involved external institutions

Universität Leipzig DE Germany (DE) Universitätsklinikum Essen DE Germany (DE) Universität Rostock DE Germany (DE)

How to cite

APA:

Bhattacharya, A., Schmitz, U., Raatz, Y., Schoenherr, M., Kottek, T., Schauer, M.,... Kunz, M. (2015). miR-638 promotes melanoma metastasis and protects melanoma cells from apoptosis and autophagy. Oncotarget, 6(5), 2966-80. https://doi.org/10.18632/oncotarget.3070

MLA:

Bhattacharya, Animesh, et al. "miR-638 promotes melanoma metastasis and protects melanoma cells from apoptosis and autophagy." Oncotarget 6.5 (2015): 2966-80.

BibTeX: Download