Guanylate binding protein 1-mediated interaction of T cell antigen receptor signaling with the cytoskeleton

Forster F, Paster W, Supper V, Schatzlmaier P, Sunzenauer S, Ostler N, Saliba A, Eckerstorfer P, Britzen-Laurent N, Schuetz G, Schmid JA, Zlabinger GJ, Naschberger E, Stürzl M, Stockinger H (2014)

Publication Type: Journal article

Publication year: 2014

Journal

Publisher: American Association of Immunologists

Book Volume: 192

Pages Range: 771-81

Journal Issue: 2

DOI: 10.4049/jimmunol.1300377

Abstract

GTPases act as important switches in many signaling events in cells. Although small and heterotrimeric G proteins are subjects of intensive studies, little is known about the large IFN-inducible GTPases. In this article, we show that the IFN-?-inducible guanylate binding protein 1 (GBP-1) is a regulator of T cell activation. Silencing of GBP-1 leads to enhanced activation of early T cell Ag receptor/CD3 signaling molecules, including Lck, that is translated to higher IL-2 production. Mass spectrometry analyses showed that regulatory cytoskeletal proteins, like plastin-2 that bundles actin fibers and spectrin ?-chain, brain 1 that links the plasma membrane to the actin cytoskeleton, are binding partners of GBP-1. The spectrin cytoskeleton influences cell spreading and surface expression of TCR/CD3 and the leukocyte phosphatase CD45. We found higher cell spreading and enhanced surface expression of TCR/CD3 and CD45 in GBP-1 silenced T cells that explain their enhanced TCR/CD3 signaling. We conclude that GBP-1 is a downstream processor of IFN-? via which T cells regulate cytoskeleton-dependent cell functions.

Authors with CRIS profile

Involved external institutions

Medizinische Universität Wien Austria (AT) Technische Universität Wien / Vienna University of Technology Austria (AT)

How to cite

APA:

Forster, F., Paster, W., Supper, V., Schatzlmaier, P., Sunzenauer, S., Ostler, N.,... Stockinger, H. (2014). Guanylate binding protein 1-mediated interaction of T cell antigen receptor signaling with the cytoskeleton. Journal of Immunology, 192(2), 771-81. https://doi.org/10.4049/jimmunol.1300377

MLA:

Forster, Florian, et al. "Guanylate binding protein 1-mediated interaction of T cell antigen receptor signaling with the cytoskeleton." Journal of Immunology 192.2 (2014): 771-81.

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