Atomic force microscopy imaging reveals the formation of ASIC/ENaC cross-clade ion channels

Jeggle P, Smith ESJ, Stewart AP, Härteis S, Korbmacher C, Edwardson JM (2015)

Publication Type: Journal article

Publication year: 2015

Journal

Biochemical and Biophysical Research Communications Elsevier

Book Volume: 464

Pages Range: 38-44

Journal Issue: 1

DOI: 10.1016/j.bbrc.2015.05.091

Abstract

ASIC and ENaC are co-expressed in various cell types, and there is evidence for a close association between them. Here, we used atomic force microscopy (AFM) to determine whether ASIC1a and ENaC subunits are able to form cross-clade hybrid ion channels. ASIC1a and ENaC could be co-isolated from detergent extracts of tsA 201 cells co-expressing the two subunits. Isolated proteins were incubated with antibodies against ENaC and Fab fragments against ASIC1a. AFM imaging revealed proteins that were decorated by both an antibody and a Fab fragment with an angle of ~120° between them, indicating the formation of ASIC1a/ENaC heterotrimers.

Authors with CRIS profile

Silke Härteis Lehrstuhl für Physiologie (Vegetative Physiologie) Christoph Korbmacher Lehrstuhl für Physiologie (Vegetative Physiologie)

Involved external institutions

University of Cambridge GB United Kingdom (GB)

How to cite

APA:

Jeggle, P., Smith, E.S.J., Stewart, A.P., Härteis, S., Korbmacher, C., & Edwardson, J.M. (2015). Atomic force microscopy imaging reveals the formation of ASIC/ENaC cross-clade ion channels. Biochemical and Biophysical Research Communications, 464(1), 38-44. https://doi.org/10.1016/j.bbrc.2015.05.091

MLA:

Jeggle, Pia, et al. "Atomic force microscopy imaging reveals the formation of ASIC/ENaC cross-clade ion channels." Biochemical and Biophysical Research Communications 464.1 (2015): 38-44.

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