In vitro effects of docosahexaenoic and eicosapentaenoic acid on human meibomian gland epithelial cells

Hampel U, Krüger M, Kunnen C, Garreis F, Willcox M, Paulsen F (2015)

Publication Type: Journal article

Publication year: 2015

Journal

Experimental Eye Research Elsevier

Book Volume: 140

Pages Range: 139-48

DOI: 10.1016/j.exer.2015.08.024

Abstract

To investigate the effect of ?-3 fatty acids on human meibomian gland epithelial cells (HMGECs, cell line) in vitro. HMGECs were stimulated with docosahexaenoic acid (DHA) or combinations with eicosapentaenoic acid (EPA) and acetyl sialic acid (ASA). Sudan III fat staining, viability and proliferation assays, electric cell-substrate impedance sensing, real-time PCR for gene expression of cyclooxygenase-2 and 15-lipoxygenase and ELISAs for resolvin D1 (RvD1), IFN?, TNF? and IL-6 were applied. Lipid droplet accumulation and viability was increased by 100 ?M DHA in the presence or absence of EPA in serum cultured HMGECs. In contrast, HMGECs cultured with DHA and EPA under serum-free conditions showed minimal lipid accumulation, decreased proliferation and viability. Normalized impedance was significantly reduced in serum-free cultured HMGECs when stimulated with DHA and EPA. HMGECs cultured in serum containing medium showed increased normalized impedance under DHA and EPA stimulation compared to DHA or EPA alone or controls. IL-6 and IFN? were downregulated in HMGECs treated for 72 h with DHA and EPA. In general, TNF?, IFN? and IL-6 levels were decreased after 72 h compared to 24 h in serum containing medium with or without DHA or EPA. The concentration of RvD1 was elevated 2-fold after DHA treatment. Cyclooxygenase-2 gene expression decreased compared to controls during DHA stimulation after 72 h. Treatment with DHA and ASA revealed a decreased 15-lipoxygenase gene expression which was reduced after three days of DHA incubation. DHA and EPA supplementation affected HMGECs in vitro and supported anti-inflammatory effects by influencing cytokine levels, decreasing COX-2 expression and increasing the production of RvD1.

Authors with CRIS profile

Ulrike Hampel Lehrstuhl für Funktionelle und Klinische Anatomie Fabian Garreis Lehrstuhl für Funktionelle und Klinische Anatomie Friedrich Paulsen Lehrstuhl für Funktionelle und Klinische Anatomie

Involved external institutions

University of New South Wales (UNSW) AU Australia (AU) University of Alabama at Birmingham (UAB) US United States (USA) (US)

How to cite

APA:

Hampel, U., Krüger, M., Kunnen, C., Garreis, F., Willcox, M., & Paulsen, F. (2015). In vitro effects of docosahexaenoic and eicosapentaenoic acid on human meibomian gland epithelial cells. Experimental Eye Research, 140, 139-48. https://doi.org/10.1016/j.exer.2015.08.024

MLA:

Hampel, Ulrike, et al. "In vitro effects of docosahexaenoic and eicosapentaenoic acid on human meibomian gland epithelial cells." Experimental Eye Research 140 (2015): 139-48.

BibTeX: Download