Thomas M, Zielke B, Reuter N, Stamminger T (2014)
Publication Type: Journal article
Publication year: 2014
Publisher: Humana Press, Inc.
Book Volume: 1119
Pages Range: 197-216
DOI: 10.1007/978-1-62703-788-4_12
One defining feature of eukaryotic cells is their compartmentalization into nucleus and cytoplasm which provides sophisticated opportunities for the regulation of gene expression. Accurate subcellular localization is crucial for the effective function of most viral macromolecules, and nuclear translocation is central to the function of herpesviral proteins that are involved in processes such as transcription or DNA replication. Human cytomegalovirus (HCMV) encodes several transactivator proteins which stimulate viral gene expression either on the transcriptional or posttranscriptional level. In this chapter, we focus on nucleocytoplasmic transport mechanisms of either proteins or RNA that are utilized during HCMV infection. We describe commonly used assays to determine the subcellular localization of a protein, its nucleocytoplasmic shuttling activity, its capacity to export unspliced RNA from the nucleus, and its association with RNA in vivo.
APA:
Thomas, M., Zielke, B., Reuter, N., & Stamminger, T. (2014). Methods to study the nucleocytoplasmic transport of macromolecules with respect to their impact on the regulation of human cytomegalovirus gene expression. Methods in Molecular Biology, 1119, 197-216. https://doi.org/10.1007/978-1-62703-788-4_12
MLA:
Thomas, Marco, et al. "Methods to study the nucleocytoplasmic transport of macromolecules with respect to their impact on the regulation of human cytomegalovirus gene expression." Methods in Molecular Biology 1119 (2014): 197-216.
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