Schrödl F, Brehmer A, Neuhuber W, Kruse F, May CA, Cursiefen C (2008)
Publication Status: Published
Publication Type: Journal article, Original article
Publication year: 2008
Publisher: Association for Research in Vision and Ophthalmology (ARVO)
Book Volume: 49
Pages Range: 5222-5229
Journal Issue: 12
DOI: 10.1167/iovs.08-1721
PURPOSE. Lymphatic vessel endothelial hyaluronic acid receptor (LYVE-1) is a newly discovered lymphatic endothelium-specific marker that is also expressed by a subpopulation of macrophages. To date, there is no report on its expression in the posterior human uvea. The purpose of this study was to investigate the expression of LYVE-1 in normal human choroids. METHODS. Eyes of body/cornea donors (55-89 years of age; 4-9 hours postmortem) were obtained. Choroids were dissected and prepared for cryosections followed by immunohistochemistry with anti-human LYVE-1 antiserum and immunogold labeling. In addition, anti-human antibodies against macrophage markers (CD68, MHC class II) and lymphatic (podoplanin) and blood vascular endothelium (CD31, vWF) were used. For documentation, light-, fluorescence-, confocal laser scanning-, and electron-microscopy were used. RESULTS. The normal human choroidal stroma contained 27 ± 86 LYVE-1 positive cells/mm2. The cells displayed irregular shapes with a relatively uniform diameter of 32 μm. Costaining with CD68 and negativity for CD31, podoplanin, and melan-A/HMB45, as well as electron microscopic features, suggest these LYVE-1 cells to be macrophages. Besides that, no classic LYVE-1/podoplanin lymphatic vessels were detected within the normal adult human choroid. CONCLUSIONS. The normal adult human choroid does not contain typical lymph vessels, but is endowed with a significant number of LYVE-1 positive macrophages. These cells may be involved in choroidal hyaluronic acid metabolism or contribute to temporary formation of lymphatic channels under inflammatory conditions. Copyright © Association for Research in Vision and Ophthalmology.
APA:
Schrödl, F., Brehmer, A., Neuhuber, W., Kruse, F., May, C.A., & Cursiefen, C. (2008). The normal human choroid is endowed with a significant number of lymphatic vessel endothelial hyaluronate receptor 1 (LYVE-1) - Positive macrophages. Investigative Ophthalmology & Visual Science, 49(12), 5222-5229. https://doi.org/10.1167/iovs.08-1721
MLA:
Schrödl, Falk, et al. "The normal human choroid is endowed with a significant number of lymphatic vessel endothelial hyaluronate receptor 1 (LYVE-1) - Positive macrophages." Investigative Ophthalmology & Visual Science 49.12 (2008): 5222-5229.
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