Plant sucrose-H+ symporters mediate the transport of vitamin H.

Ludwig A, Stolz J, Sauer N (2000)


Publication Language: English

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2000

Journal

Book Volume: 24

Pages Range: 503-509

Journal Issue: 4

DOI: 10.1111/j.1365-313X.2000.00900.x

Open Access Link: http://onlinelibrary.wiley.com/doi/10.1111/j.1365-313X.2000.00900.x/abstract

Abstract

A cDNA coding for a vitamin H (biotin) transport protein from Arabidopsis was identified by genetic complementation of a biotin uptake-deficient yeast mutant. Vitamin H transport by this protein was sensitive to the SH-group inhibitor p-chloromercuribenzene sulfonic acid (PCMBS) and to the uncoupler carbonyl cyanide-m-chlorophenylhydrazone (CCCP), suggesting an energy-dependent biotin-H+ symport mechanism. The transport activity could contribute to the so-far uncharacterized plant sucrose-H+ symporter AtSUC5 which mediates the energy-dependent transport of biotin and sucrose, and restores growth of the biotin transport-deficient yeast mutant on medium with low biotin concentrations. Functional comparison of the AtSUC5 transporter with previously characterized plant sucrose or monosaccharide transporters revealed that biotin transport may be a general and specific property of all plant sucrose transporters (sucrose/biotin-H+ symporters). This first report on a transporter with dual substrate specificity for two structurally unrelated molecules has a major impact on general thinking concerning the specificity of membrane transporters. The physiological relevance of this finding is discussed.

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How to cite

APA:

Ludwig, A., Stolz, J., & Sauer, N. (2000). Plant sucrose-H+ symporters mediate the transport of vitamin H. Plant Journal, 24(4), 503-509. https://doi.org/10.1111/j.1365-313X.2000.00900.x

MLA:

Ludwig, Andreas, Jürgen Stolz, and Norbert Sauer. "Plant sucrose-H+ symporters mediate the transport of vitamin H." Plant Journal 24.4 (2000): 503-509.

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