Defective autophagosome formation in p53-null colorectal cancer reinforces crocin-induced apoptosis
Amin A, Bajbouj K, Koch AM, Gandesiri M, Schneider-Stock R (2015)
Publication Type: Journal article
Publication year: 2015
Journal
Book Volume: 16
Pages Range: 1544-61
Journal Issue: 1
DOI: 10.3390/ijms16011544
Abstract
Crocin, a bioactive molecule of saffron, inhibited proliferation of both HCT116 wild-type and HCT116 p53(-/-) cell lines at a concentration of 10 mM. Flow cytometric analysis of cell cycle distribution revealed that there was an accumulation of HCT116 wild-type cells in G1 (55.9%, 56.1%) compared to the control (30.4%) after 24 and 48 h of crocin treatment, respectively. However, crocin induced only mild G2 arrest in HCT116 p53(-/-) after 24 h. Crocin induced inefficient autophagy in HCT116 p53(-/-) cells, where crocin induced the formation of LC3-II, which was combined with a decrease in the protein levels of Beclin 1 and Atg7 and no clear p62 degradation. Autophagosome formation was not detected in HCT116 p53(-/-) after crocin treatment predicting a nonfunctional autophagosome formation. There was a significant increase of p62 after treating the cells with Bafilomycin A1 (Baf) and crocin compared to crocin exposure alone. Annexin V staining showed that Baf-pretreatment enhanced the induction of apoptosis in HCT116 wild-type cells. Baf-exposed HCT116 p53(-/-) cells did not, however, show any enhancement of apoptosis induction despite an increase in the DNA damage-sensor accumulation, ?H2AX indicating that crocin induced an autophagy-independent classical programmed cell death.
Authors with CRIS profile
Anna Maria Koch
Department of Paediatric Cardiac Surgery
Regine Schneider-Stock
Professur für Experimentelle Tumorpathologie
Involved external institutions
United Arab Emirates (AE)How to cite
APA:
Amin, A., Bajbouj, K., Koch, A.M., Gandesiri, M., & Schneider-Stock, R. (2015). Defective autophagosome formation in p53-null colorectal cancer reinforces crocin-induced apoptosis. International Journal of Molecular Sciences, 16(1), 1544-61. https://doi.org/10.3390/ijms16011544
MLA:
Amin, Amr, et al. "Defective autophagosome formation in p53-null colorectal cancer reinforces crocin-induced apoptosis." International Journal of Molecular Sciences 16.1 (2015): 1544-61.
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