Jungbauer S, Buehler PK, Neubauer J, Haas C, Heitzmann D, Tegtmeier I, Sterner C, Barhanin J, Georgieff M, Warth R, Thomas J (2016)
Publication Type: Journal article
Publication year: 2016
DOI: 10.1016/j.resp.2016.11.005
TASK-1 potassium channels have been implicated in central and peripheral chemoreception; however, the precise contribution of TASK-1 for the control of respiration is still under debate. Here, we investigated the respiration of unrestrained adult and neonatal TASK-1 knockout mice (TASK-1(-/-)) using a plethysmographic device. Respiration in adult female TASK-1(-/-) mice under control (21% O2), hypoxia and hypercapnia was unaffected. Under acute hypoxia male TASK-1(-/-) mice exhibited a reduced increase of the respiratory frequency (fR) compared to wildtypes. However, the tidal volume (VT) of male TASK-1(-/-) mice was strongly enhanced. The volatile anesthetic isoflurane induced in male TASK-1(-/-) and male wild type mice (TASK-1(+/+)) a similar respiratory depression. Neonatal TASK-1(-/-) mice demonstrated a 30-40% decrease of the minute volume, caused by a reduction of the fR under control condition (21% O2). Under hypoxia, neonatal TASK-1(-/-) mice more frequently stopped breathing (apnea>3s) suggesting an increased hypoxia-sensitivity. As reported before, this increased hypoxia sensitivity had no influence on the survival rate of neonatal TASK-1(-/-) mice. In adult and neonatal mice, TASK-1 gene deletion induced a significant prolongation of the relaxation time (RT), which is a parameter for expiration kinetics. Additionally, screening for mutations in the human TASK-1 gene in 155 cases of sudden infant death syndrome (SIDS) was inconclusive. In conclusion, these data are suggestive for an increased hypoxia-sensitivity of neonatal TASK-1(-/-) mice, however, without causing an increase in neonatal lethality. In adult female TASK-1(-/-) mice respiration was unaffected, whereas adult male TASK-1(-/-) mice showed a modified breathing pattern. These results are suggestive for sex-specific mechanisms for compensating the inactivation of TASK-1 in mice.
APA:
Jungbauer, S., Buehler, P.K., Neubauer, J., Haas, C., Heitzmann, D., Tegtmeier, I.,... Thomas, J. (2016). Sex-dependent differences in the in vivo respiratory phenotype of the TASK-1 potassium channel knockout mouse. Respiratory Physiology & Neurobiology. https://dx.doi.org/10.1016/j.resp.2016.11.005
MLA:
Jungbauer, Stefan, et al. "Sex-dependent differences in the in vivo respiratory phenotype of the TASK-1 potassium channel knockout mouse." Respiratory Physiology & Neurobiology (2016).
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