Eberhardt E, Havlicek S, Schmidt D, Link A, Neacsu C, Kohl Z, Hampl M, Kist A, Klinger A, Nau C, Schüttler J, Alzheimer C, Winkler J, Namer B, Winner B, Lampert A (2015)
Publication Type: Journal article
Publication year: 2015
Book Volume: 5
Pages Range: 305-13
Journal Issue: 3
DOI: 10.1016/j.stemcr.2015.07.010
Human pluripotent stem cells (hPSCs) offer the opportunity to generate neuronal cells, including nociceptors. Using a chemical-based approach, we generated nociceptive sensory neurons from HUES6 embryonic stem cells and retrovirally reprogrammed induced hPSCs derived from fibroblasts. The nociceptive neurons expressed respective markers and showed tetrodotoxin-sensitive (TTXs) and -resistant (TTXr) voltage-gated sodium currents in patch-clamp experiments. In contrast to their counterparts from rodent dorsal root ganglia, TTXr currents of hPSC-derived nociceptors unexpectedly displayed a significantly more hyperpolarized voltage dependence of activation and fast inactivation. This apparent discrepancy is most likely due to a substantial expression of the developmentally important sodium channel NAV1.5. In view of the obstacles to recapitulate neuropathic pain in animal models, our data advance hPSC-derived nociceptors as a better model to study developmental and pathogenetic processes in human nociceptive neurons and to develop more specific small molecules to attenuate pain.
APA:
Eberhardt, E., Havlicek, S., Schmidt, D., Link, A., Neacsu, C., Kohl, Z.,... Lampert, A. (2015). Pattern of Functional TTX-Resistant Sodium Channels Reveals a Developmental Stage of Human iPSC- and ESC-Derived Nociceptors. Stem Cell Reports, 5(3), 305-13. https://doi.org/10.1016/j.stemcr.2015.07.010
MLA:
Eberhardt, Esther, et al. "Pattern of Functional TTX-Resistant Sodium Channels Reveals a Developmental Stage of Human iPSC- and ESC-Derived Nociceptors." Stem Cell Reports 5.3 (2015): 305-13.
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