Danzer CA, Koller A, Baier J, Arnold H, Giessler C, Opoka R, Schmidt S, Willers M, Mihai S, Parsch H, Wirtz S, Daniel C, Reinhold A, Engelmann S, Kliche S, Bogdan C, Hoebe K, Mattner J (2016)
Publication Type: Journal article
Publication year: 2016
Book Volume: 46
Pages Range: 2121-36
Journal Issue: 9
TCR ligation is critical for the selection, activation, and integrin expression of T lymphocytes. Here, we explored the role of the TCR adaptor protein slp-76 on iNKT-cell biology. Compared to B6 controls, slp-76(ace/ace) mice carrying a missense mutation (Thr428Ile) within the SH2-domain of slp-76 showed an increase in iNKT cells in the thymus and lymph nodes, but a decrease in iNKT cells in spleens and livers, along with reduced ADAP expression and cytokine response. A comparable reduction in iNKT cells was observed in the livers and spleens of ADAP-deficient mice. Like ADAP(-/-) iNKT cells, slp-76(ace/ace) iNKT cells were characterized by enhanced CD11b expression, correlating with an impaired induction of the TCR immediate-early gene Nur77 and a decreased adhesion to ICAM-1. Furthermore, CD11b-intrinsic effects inhibited cytokine release, concanavalin A-mediated inflammation, and iNKT-cell accumulation in the liver. Unlike B6 and ADAP(-/-) mice, the expression of the transcription factors Id3 and PLZF was reduced, whereas NP-1-expression was enhanced in slp-76(ace/ace) mice. Blockade of NP-1 decreased the recovery of iNKT cells from peripheral lymph nodes, identifying NP-1 as an iNKT-cell-specific adhesion factor. Thus, slp-76 contributes to the regulation of the tissue distribution, PLZF, and cytokine expression of iNKT cells via ADAP-dependent and -independent mechanisms.
APA:
Danzer, C.A., Koller, A., Baier, J., Arnold, H., Giessler, C., Opoka, R.,... Mattner, J. (2016). A mutation within the SH2 domain of slp-76 regulates the tissue distribution and cytokine production of iNKT cells in mice. European Journal of Immunology, 46(9), 2121-36. https://doi.org/10.1002/eji.201646331
MLA:
Danzer, Claudia Agnes, et al. "A mutation within the SH2 domain of slp-76 regulates the tissue distribution and cytokine production of iNKT cells in mice." European Journal of Immunology 46.9 (2016): 2121-36.
BibTeX: Download