Koch S, Reppert S, Finotto S (2015)
Publication Type: Journal article
Publication year: 2015
Book Volume: 45
Pages Range: 1356-66
Journal Issue: 8
DOI: 10.1111/cea.12493
NFATc1 isoforms are highly regulated in peripheral T cells where they contribute to the effector function and cell homeostasis.Here, we investigated the role of NFATc1 in asthma and in T cells.In a murine model of allergic asthma, we analysed differences in T-cell development in this allergic disease model, between wild-type and NFATc1 conditional knockout mice. Thus, we performed quantitative real-time PCR to investigate the mRNA expression of Th2-associated genes as well as genes that are involved in IgE immunoglobulin class-switch. Additionally, we used ELISA, Western blot and flow cytometry (FACS) to analyse protein concentrations of Th1-, Th2- and Th17-specific transcription factors and cytokines and the Th2 chemokine, thymus and activation-regulated chemokine/chemokine ligand 17 (TARC/CCL17) by ELISA.Mice lacking NFATc1 in CD4(+) T cells display a significant reduction in lung Th2 and Th17 as well as an increase of Th1 cells in an experimental asthma model. Additionally, Batf gene, a recently described transcription factor of the Th2 and Th17 cell differentiation as well as a key T and B transcription factor involved in the IgE immunoglobulin class-switch, was found decreased in the lungs of these mice. As a consequence, serum OVA-specific IgE and IgG1 levels were found significantly decreased after allergen exposure and in the absence of NFATc1 in T cells in experimental allergic asthma.Targeting NFATc1 in T lymphocytes ameliorated the allergic trait in the airways of NFATc1(fl/fl) xCD4Cre mice. NFATc1 emerges as a novel target for anti-allergy intervention.
APA:
Koch, S., Reppert, S., & Finotto, S. (2015). NFATc1 deletion in T lymphocytes inhibits the allergic trait in a murine model of asthma. Clinical and Experimental Allergy, 45(8), 1356-66. https://doi.org/10.1111/cea.12493
MLA:
Koch, S., S. Reppert, and Susetta Finotto. "NFATc1 deletion in T lymphocytes inhibits the allergic trait in a murine model of asthma." Clinical and Experimental Allergy 45.8 (2015): 1356-66.
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