Graf L, Feichtinger S, Naing Z, Hutterer C, Milbradt J, Webel R, Wagner S, Scott GM, Hamilton ST, Rawlinson WD, Stamminger T, Thomas M, Marschall M (2016)
Publication Type: Journal article
Publication year: 2016
Book Volume: 97
Pages Range: 144-51
Journal Issue: 1
DOI: 10.1099/jgv.0.000337
Cyclin-dependent kinases (CDKs) are multifaceted regulators involved in the replication of human cytomegalovirus. Recently, we demonstrated an interaction of CDK9-cyclin T1 as well as viral CDK orthologue pUL97 with the viral regulator pUL69, thereby leading to pUL69-activating phosphorylation. Here, we demonstrate that colocalization and direct pUL69-cyclin T1 interaction is independent of viral strains and host cell types. In vitro phosphorylation of pUL69 by CDK9 or pUL97 did not occur in a single site-specific manner, but at multiple sites. The previously described fine-speckled nuclear aggregation of pUL69 was assigned to the late phase of viral replication. CDK inhibitors, including a novel inhibitor of the CDK-activating kinase CDK7, massively intensified this fine-speckled accumulation. Interestingly, we also observed spontaneous pUL69 accumulation in the absence of inhibitors at a lower frequency. These findings provide new insight into pUL69 kinase interregulation and emphasize the importance of pUL69 phosphorylation for correct intranuclear localization.
APA:
Graf, L., Feichtinger, S., Naing, Z., Hutterer, C., Milbradt, J., Webel, R.,... Marschall, M. (2016). New insight into the phosphorylation-regulated intranuclear localization of human cytomegalovirus pUL69 mediated by cyclin-dependent kinases (CDKs) and viral CDK orthologue pUL97. Journal of General Virology, 97(1), 144-51. https://doi.org/10.1099/jgv.0.000337
MLA:
Graf, Laura, et al. "New insight into the phosphorylation-regulated intranuclear localization of human cytomegalovirus pUL69 mediated by cyclin-dependent kinases (CDKs) and viral CDK orthologue pUL97." Journal of General Virology 97.1 (2016): 144-51.
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