Nakanomyo I, Kost B, Chua NH, Fukuda H (2002)
Publication Status: Published
Publication Type: Journal article
Publication year: 2002
Publisher: Oxford University Press (OUP): Policy B - Oxford Open Option B
Book Volume: 43
Pages Range: 1484-1492
Journal Issue: 12
DOI: 10.1093/pcp/pcf170
Rac-type small GTPases are known to function in some cellular processes in plants. To further understand the involvement of Rac type GTPases in plant development, we isolated from cultured Zinnia cells a gene (ZeRAC2) encoding a new Rac-type small GTPase. ZeRAC2 mPNA accumulates preferentially in xylogenic culture and transiently at the time when visible tracheary elements appear. Experiments with ZeRAC2 recombinant proteins demonstrated that ZeRAC2 binds to and hydrolyzes GTP. A GFP-ZeRAC2 fusion protein was localized to the plasma membrane. Together with the fact that ZeRAC2 possesses a putative geranylgeranylation site at the C-terminus, this suggests that ZeRAC2 acts on the plasma membrane. In situ hybridization indicated that ZeRAC2 mRNA accumulates preferentially in xylem parenchyma and tracheary element precursor cells, and surprisingly the accumulation is restricted to the site facing developing tracheary elements.
APA:
Nakanomyo, I., Kost, B., Chua, N.-H., & Fukuda, H. (2002). Preferential and asymmetrical accumulation of a Rac small GTPase mRNA in differentiating xylem cells of Zinnia elegans. Plant and Cell Physiology, 43(12), 1484-1492. https://dx.doi.org/10.1093/pcp/pcf170
MLA:
Nakanomyo, Ikuko, et al. "Preferential and asymmetrical accumulation of a Rac small GTPase mRNA in differentiating xylem cells of Zinnia elegans." Plant and Cell Physiology 43.12 (2002): 1484-1492.
BibTeX: Download